FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-ZBTB48 antibody (AAA126619).Overlay histogram showing SiHa cells stained with AAA126619 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB48 Antibody (AAA126619, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human ZBTB48 Polyclonal Antibody | anti-ZBTB48 antibody
Anti-ZBTB48 Antibody Picoband
Gene Names
ZBTB48; HKR3; ZNF855; pp9964
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
ZBTB48, Antibody; Anti-ZBTB48 Antibody Picoband; anti-ZBTB48 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-ZBTB48 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human ZBTB48 recombinant protein (Position: M1-M623).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-ZBTB48 antibody (AAA126619).Overlay histogram showing SiHa cells stained with AAA126619 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB48 Antibody (AAA126619, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-ZBTB48 antibody (AAA126619).Overlay histogram showing SiHa cells stained with AAA126619 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB48 Antibody (AAA126619, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of ZBTB48 using anti-ZBTB48 antibody (AAA126619).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB48 antigen affinity purified polyclonal antibody (#AAA126619) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZBTB48 at approximately 77 kDa. The expected band size for ZBTB48 is at 77 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of ZBTB48 using anti-ZBTB48 antibody (AAA126619).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB48 antigen affinity purified polyclonal antibody (#AAA126619) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZBTB48 at approximately 77 kDa. The expected band size for ZBTB48 is at 77 kDa.)
Related Product Information for anti-ZBTB48 antibody
Zinc finger and BTB domain containing 48 (ZBTB48), also known as telomeric zinc-finger associated protein (TZAP), is a protein that directly binds to the double-stranded repeat sequence of telomeres. In humans it is encoded by the ZBTB48 gene. ZBTB48, also named as TZAP and HKR3, is a 688 amino acid protein, which contains 1 BTB (POZ) domain and 11 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZBTB48 is detected in adrenal gland and neuroblastoma. ZBTB48 binds to and regulates the J and/or S elements in MHC II promoter. Zinc finger and BTB domain containing 48 (ZBTB48), also known as telomeric zinc-finger associated protein (TZAP), is a protein that triggers the trimming of telomeres in the absence of shelterin. ZBTB48, is a relatively uncharacterized POK family protein with a POZ-domain and 11 zinc finger domains. ZBTB48, is ubiquitously expressed in human tissues. The ZBTB48 gene is mapped to human chromosome 1p36, which is commonly rearranged (leiomyoma and leukemias) or deleted in various cancers (neuroblastoma, melanoma, Merkel cell carcinomas, pheochromocytoma and breast and colon carcinomas. A correlation between deletions or rearrangements of HKR3 and human cancer suggest a role for ZBTB48 as a potential tumor suppressor.
Product Categories/Family for anti-ZBTB48 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,054 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 48
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 48
NCBI Official Symbol
ZBTB48
NCBI Official Synonym Symbols
HKR3; ZNF855; pp9964
NCBI Protein Information
zinc finger and BTB domain-containing protein 48
UniProt Protein Name
Zinc finger and BTB domain-containing protein 48
UniProt Gene Name
ZBTB48
UniProt Synonym Gene Names
HKR3; ZNF855
UniProt Entry Name
ZBT48_HUMAN
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Product Notes
The ZBTB48 zbtb48 (Catalog #AAA126619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZBTB48 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB48 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the ZBTB48 zbtb48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB48, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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