Rabbit ZIPK Polyclonal Antibody | anti-DAPK3 antibody

ZIPK Antibody

Cat. Num. AAA10927

• Gene Names: DAPK3; DLK; ZIP; ZIPK
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ZIPK Antibody is affinity chromatography purified via peptide column.
• Synonyms: ZIPK; Polyclonal Antibody; ZIPK Antibody; ZIP; Death-associated protein kinase 3; DAP-like kinase; DAP kinase 3; death-associated protein kinase 3; anti-DAPK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ZIPK Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 454

• Applicable Applications for anti-DAPK3 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 mug/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
• Buffer: ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ZIPK antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ICC (Immunocytochemistry)

(Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.Green: ZIPK Antibody (2067)Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 μg/mL.)

WB (Western Blot)

(Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.)

Related Product Information for anti-DAPK3 antibody

ZIPK Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 5162884
• NCBI GeneID: 1613
• UniProt Accession #: O43293; Q05JY4; A0AVN4; B3KQE2
• Molecular Weight: 40 kDa
• NCBI Official Full Name: ZIP kinase
• NCBI Official Synonym Full Names: death-associated protein kinase 3
• NCBI Official Symbol: DAPK3
• NCBI Official Synonym Symbols: DLK; ZIP; ZIPK
• NCBI Protein Information: death-associated protein kinase 3; ZIP-kinase; DAP kinase 3; MYPT1 kinase; DAP-like kinase; zipper-interacting protein kinase
• UniProt Protein Name: Death-associated protein kinase 3
• UniProt Gene Name: DAPK3
• UniProt Synonym Gene Names: ZIPK; DAP kinase 3; Dlk; ZIP-kinase
• UniProt Entry Name: DAPK3_HUMAN