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IF (Immunofluorescence)
(Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody (Center). MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.)
Rabbit anti-Human ZNF202 Polyclonal Antibody | anti-ZNF202 antibody
ZNF202 Antibody (Center)
Gene Names
ZNF202; ZSCAN42; ZKSCAN10
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZNF202, Antibody; ZNF202 Antibody (Center); Zinc finger protein 202; Zinc finger protein with KRAB and SCAN domains 10; ZNF202; ZKSCAN10; anti-ZNF202 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZNF202 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-370 amino acids from the Central region of human ZNF202.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Positions
342-370
Sequence Length
424
Applicable Applications for anti-ZNF202 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody (Center). MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody (Center). MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ZNF202 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ZNF202 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(ZNF202 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ZNF202 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(ZNF202 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF202 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ZNF202 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF202 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ZNF202 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ZNF202 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ZNF202 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ZNF202 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ZNF202 antibody
ZNF202 (Zinc finger protein 202) is a transcriptional repressor of genes affecting the vascular endothelium as well as lipid metabolism and energy homeostasis. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.
References
Aberg, K., et al. Biol. Psychiatry 67(3):279-282(2010)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)
Patterson, E.S., et al. Physiol. Genomics 34(3):277-284(2008)
Stene, M.C., et al. J. Am. Coll. Cardiol. 52(5):369-377(2008)
Stene, M.C., et al. J. Lipid Res. 47(5):944-952(2006)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)
Patterson, E.S., et al. Physiol. Genomics 34(3):277-284(2008)
Stene, M.C., et al. J. Am. Coll. Cardiol. 52(5):369-377(2008)
Stene, M.C., et al. J. Lipid Res. 47(5):944-952(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74720
NCBI Official Full Name
zinc finger protein 202 isoform 1
NCBI Official Synonym Full Names
zinc finger protein 202
NCBI Official Symbol
ZNF202
NCBI Official Synonym Symbols
ZSCAN42; ZKSCAN10
NCBI Protein Information
zinc finger protein 202
UniProt Protein Name
Zinc finger protein 202
UniProt Gene Name
ZNF202
UniProt Synonym Gene Names
ZKSCAN10
UniProt Entry Name
ZN202_HUMAN
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Product Notes
The ZNF202 znf202 (Catalog #AAA289807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 342-370. The ZNF202 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF202 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ZNF202 znf202 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF202, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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