Standard Curve (Sample)
Porcine CREB-regulated transcription coactivator 3 (CRTC3) ELISA Kit | CRTC3 elisa kit
Porcine CREB-regulated transcription coactivator 3 (CRTC3) ELISA Kit
Gene Names
CRTC3; TORC3; TORC-3
Reactivity
Porcine
Synonyms
CREB-regulated transcription coactivator 3 (CRTC3); N/A; Porcine CREB-regulated transcription coactivator 3 (CRTC3) ELISA Kit; CRTC3 elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This CRTC3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CRTC3. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CRTC3 elisa kit
Principle of the assay: CRTC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRTC3 antibody and an CRTC3-HRP conjugate. The assay sample and buffer are incubated together with CRTC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRTC3 concentration since CRTC3 from samples and CRTC3-HRP conjugate compete for the anti-CRTC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRTC3 from the sample, fewer sites are left to bind CRTC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRTC3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CRTC3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,830 Da
NCBI Official Full Name
CREB-regulated transcription coactivator 3 isoform a
NCBI Official Synonym Full Names
CREB regulated transcription coactivator 3
NCBI Official Symbol
CRTC3
NCBI Official Synonym Symbols
TORC3; TORC-3
NCBI Protein Information
CREB-regulated transcription coactivator 3; transducer of regulated CREB protein 3; transducer of regulated cAMP response element-binding protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3
UniProt Protein Name
CREB-regulated transcription coactivator 3
UniProt Gene Name
CRTC3
UniProt Synonym Gene Names
TORC3; TORC-3; Transducer of CREB protein 3
UniProt Entry Name
CRTC3_HUMAN
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Product Notes
The Porcine CRTC3 crtc3 (Catalog #AAA245475) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245475 ELISA Kit recognizes Porcine CRTC3. It is sometimes possible for the material contained within the vial of "CREB-regulated transcription coactivator 3 (CRTC3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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