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product-image-AAA42737_SC15.jpg Standard Curve (Sample)

Porcine Epidermal growth factor, EGF ELISA Kit | EGF elisa kit

Porcine Epidermal growth factor, EGF ELISA Kit

Gene Names
EGF; URG; HOMG4
Reactivity
Porcine
Synonyms
Epidermal growth factor, EGF; N/A; Porcine Epidermal growth factor, EGF ELISA Kit; EGF elisa kit
Ordering
Reactivity
Porcine
Sequence Length
53
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
25-1600ng/L
Sensitivity
13.93ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Standard Curve (Sample)

product-image-AAA42737_SC15.jpg Standard Curve (Sample)
Related Product Information for EGF elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine EGF antibody. EGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine EGF Antibody is added and binds to EGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine EGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Epidermal growth factor (also known as EGF) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
129,256 Da
NCBI Official Full Name
epidermal growth factor, partial
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
UniProt Gene Name
EGF
UniProt Synonym Gene Names
EGF

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Product Notes

The Porcine EGF egf (Catalog #AAA42737) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA42737 ELISA Kit recognizes Porcine EGF. It is sometimes possible for the material contained within the vial of "Epidermal growth factor, EGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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