Application Data
(Recombinant PRDM9 protein activity assay. PRDM9 protein was used in an HMT assay to determine enzyme acticvity. Activity was detected by MALDI-TOF.)
PRDM9 recombinant protein
Recombinant PRDM9 (191-414) protein
Gene Names
PRDM9; PFM6; MSBP3; ZNF899; MEISETZ
Synonyms
PRDM9; N/A; Recombinant PRDM9 (191-414) protein; PFM6; MSBP3; ZNF899; MEISETZ; PRDM9 recombinant protein
Host
E Coli
Form/Format
Recombinant PRDM9 protein was expressed in E Coli and is supplied in 20mM Tris pH7.5, 300mM NaCl, 0.5mM TCEP, 5% glycerol, 1 uM ZnCl2.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant PRDM9 is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 50mM TrisCl, pH 8.5, 30mM NaCl, 5% glycerol, 2mM DTT, 5, 25, and 50uM H3 (1-24) peptide, 100nm Recombinant PRMT5 protein at room temperature.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant PRDM9 protein activity assay. PRDM9 protein was used in an HMT assay to determine enzyme acticvity. Activity was detected by MALDI-TOF.)
Application Data
(Recombinant PRDM9 protein activity assay. PRDM9 protein was used in an HMT assay to determine enzyme acticvity. Activity was detected by MALDI-TOF.)
SDS-PAGE
(Recombinant PRDM9 protein gel. PRDM9 protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant PRDM9 protein gel. PRDM9 protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for PRDM9 recombinant protein
Short Description: Recombinant PRDM9 protein contains amino acids 191-414 of the human PRDM9 (accession number NP_064612.2) with a GST-Tag and expressed in E Coli with a molecular weight of 25.6 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: PRDM9 (PR Domain Containing 9) is a Histone methyltransferase that specifically trimethylates Lys-4 of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does has the ability to mono- and dimethylate Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity).
Background: PRDM9 (PR Domain Containing 9) is a Histone methyltransferase that specifically trimethylates Lys-4 of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does has the ability to mono- and dimethylate Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity).
Product Categories/Family for PRDM9 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,376 Da
NCBI Official Full Name
histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
NCBI Official Synonym Full Names
PR domain 9
NCBI Official Symbol
PRDM9
NCBI Official Synonym Symbols
PFM6; MSBP3; ZNF899; MEISETZ
NCBI Protein Information
histone-lysine N-methyltransferase PRDM9
Similar Products
Product Notes
The PRDM9 (Catalog #AAA59728) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRDM9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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