Application Data
PTC2 recombinant protein
PTC2 Mutant (PRKAR1A-RET), Active
Gene Names
PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
Applications
Western Blot
Synonyms
PTC2; N/A; PTC2 Mutant (PRKAR1A-RET), Active; PTC2 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
PRKAR1A (1-236)-RET (713-end)
Sequence Length
4325
Applicable Applications for PTC2 recombinant protein
WB (Western Blot), Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PTC2 recombinant protein
Description: Recombinant human RET/PTC2, the fusion protein [PRKAR1A (1-236)-RET (713-end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: RET/PTC2 is fused of the tyrosine-kinase domain of proto-RET with the regulatory subunit R/A of c-AMP-dependent protein kinase A, by transforming a chromosomal translocation at t (10;17) (q11.2;q23) (1). The RET/PTC oncoproteins display constitutive TK activity and tyrosine phosphorylation. The RET/PTC2 Tyr-539 is an essential docking site for activating the SH2-containing transducer phospholipase PLCgamma (2). The RET/PTC2 activation may play crucial roles in papillary thyroid tumorigenesis and neoplastic oncogene.
Scientific Background: RET/PTC2 is fused of the tyrosine-kinase domain of proto-RET with the regulatory subunit R/A of c-AMP-dependent protein kinase A, by transforming a chromosomal translocation at t (10;17) (q11.2;q23) (1). The RET/PTC oncoproteins display constitutive TK activity and tyrosine phosphorylation. The RET/PTC2 Tyr-539 is an essential docking site for activating the SH2-containing transducer phospholipase PLCgamma (2). The RET/PTC2 activation may play crucial roles in papillary thyroid tumorigenesis and neoplastic oncogene.
Product Categories/Family for PTC2 recombinant protein
References
1. Gabriella S. et al: A t (10; 17) translocation creates the RET/PTC2 chimeric transforming sequence in papillary thyroid carcinoma. Genes, Chromosomes and Cancer. 9:244-250 (1994). 2. M G Borrello, et al: The full oncogenic activity of Ret/ptc2 depends on tyrosine 539, a docking site for phospholipase Cgamma. Mol Cell Biol. May 1996; 16 (5): 2151 -2163.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~105 kDa
NCBI Official Full Name
Homo sapiens protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
protein kinase cAMP-dependent type I regulatory subunit alpha
NCBI Official Symbol
PRKAR1A
NCBI Official Synonym Symbols
CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
NCBI Protein Information
cAMP-dependent protein kinase type I-alpha regulatory subunit
UniProt Protein Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
UniProt Gene Name
PRKAR1A
UniProt Synonym Gene Names
PKR1; PRKAR1; TSE1; TSE1
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Product Notes
The PTC2 prkar1a (Catalog #AAA73110) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is PRKAR1A (1-236)-RET (713-end). AAA Biotech's PTC2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the PTC2 prkar1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTC2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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