Application Data
(HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
PRKCB recombinant protein
Recombinant PRKCB protein
Application Data
(HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant PRKCB protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 78.3kDa Purity: >95%)
SDS-PAGE
(Recombinant PRKCB protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 78.3kDa Purity: >95%)
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Product Notes
The PRKCB (Catalog #AAA60414) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRKCB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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