DNA-dependent protein kinase catalytic subunit Recombinant Protein | PRKDC recombinant protein

Recombinant Mouse DNA-dependent protein kinase catalytic subunit

Cat. Num. AAA113586

• Gene Names: Prkdc; p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-dependent protein kinase catalytic subunit; N/A; Recombinant Mouse DNA-dependent protein kinase catalytic subunit; p460; PRKDC recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 3747-4015aa; Partial
• Sequence: EYPFLVKGGEDLRQDQRIEQIFEVMNAILSQDAACSQRNMQLRTYRVVPMTSRLGLIEWIENTMTLKDLLLSNMSQEEKVANNSDPKAPIRDYKDWLMKVSGKSDAGAYVLMYSRANRTETVVAFRRRESQVPPDLLKRAFVKMSTSPEAFLALRSHFASSHALLCISHWLLGIGDRHLNNFMVAMETGSVIGIDFGHAFGSATQFLPVPELMPFRLTRQFVSLMLPMKETGLMCTVMVHALRAFRSCAGLLTDTMEIFVKEPSFDWKS
• Sequence Length: 4128

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for PRKDC recombinant protein

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism.

References

Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice.Araki R., Fujimori A., Hamatani K., Mita K., Saito T., Mori M., Fukumura R., Morimyo M., Muto M., Itoh M., Tatsumi K., Abe M.Proc. Natl. Acad. Sci. U.S.A. 94:2438-2443(1997) Murine cell line SX9 bearing a mutation in the DNA-PKcs gene exhibits aberrant V(D) J recombination not only in the coding joint but also in the signal joint.Fukumura R., Araki R., Fujimori A., Mori M., Saito T., Watanabe F., Sarashi M., Itsukaichi H., Eguch-Kasai K., Sato K., Tatsumi K., Abe M.J. Biol. Chem. 273:13058-13064(1998) The murine DNA-PKcs gene consists of 86 exons dispersed in more than 250 kb.Fujimori A., Araki R., Fukumura R., Saito T., Mori M., Mita K., Tatsumi K., Abe M.Genomics 45:194-199(1997) Lineage-specific biology revealed by a finished genome assembly of the mouse.Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) Mouse Cdc21 only 0.5 kb upstream from DNA-PKcs in a head-to-head organization an implication of co-evolution of ATM family members and cell cycle regulating genes.Saito T., Matsuda Y., Ishii H., Watanabe F., Mori M., Hayashi A., Araki R., Fujimori A., Fukumura R., Morimyo M., Tatsumi K., Hori T., Abe M.Mamm. Genome 9:769-772(1998) Nonhomologous end-joining deficiency of L5178Y-S cells is not associated with mutation in the ABCDE autophosphorylation cluster.Brzoska K., Kruszewski M., Szumiel I.Acta Biochim. Pol. 53:233-236(2006) Cloning and chromosomal mapping of the mouse DNA-dependent protein kinase gene.Hamatani K., Matsuda Y., Araki R., Itoh M., Abe M.Immunogenetics 45:1-5(1996) Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse.Blunt T., Gell D., Fox M., Taccioli G.E., Lehmann A.R., Jackson S.P., Jeggo P.A.Proc. Natl. Acad. Sci. U.S.A. 93:10285-10290(1996) Biochemical and genetic defects in the DNA-dependent protein kinase in murine scid lymphocytes.Danska J.S., Holland D.P., Mariathasan S., Williams K.M., Guidos C.J.Mol. Cell. Biol. 16:5507-5517(1996) Functional interaction between DNA-PK and c-Abl in response to DNA damage.Kharbanda S., Pandey P., Jin S., Inoue S., Bharti A., Yuan Z.-M., Weichselbaum R., Weaver D., Kufe D.Nature 386:732-735(1997) Functional interaction between DNA-PKcs and telomerase in telomere length maintenance.Espejel S., Franco S., Sgura A., Gae D., Bailey S.M., Taccioli G.E., Blasco M.A.EMBO J. 21:6275-6287(2002) GAPDH mediates nitrosylation of nuclear proteins.Kornberg M.D., Sen N., Hara M.R., Juluri K.R., Nguyen J.V., Snowman A.M., Law L., Hester L.D., Snyder S.H.Nat. Cell Biol. 12:1094-1100(2010) Phosphorylation of the cryptochrome 1 C-terminal tail regulates circadian period length.Gao P., Yoo S.H., Lee K.J., Rosensweig C., Takahashi J.S., Chen B.P., Green C.B.J. Biol. Chem. 288:35277-35286(2013)

NCBI and Uniprot Product Information

• NCBI GI #: 124517706
• NCBI GeneID: 19090
• NCBI Accession #: NP_035289.2
• NCBI GenBank Nucleotide #: NM_011159.2
• UniProt Accession #: P97313; O88187; P97928; Q307W9; Q3V2W8; Q8C2A7; Q9Z341; E9QN15
• Molecular Weight: 34.6 kDa
• NCBI Official Full Name: DNA-dependent protein kinase catalytic subunit
• NCBI Official Synonym Full Names: protein kinase, DNA activated, catalytic polypeptide
• NCBI Official Symbol: Prkdc
• NCBI Official Synonym Symbols: p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
• NCBI Protein Information: DNA-dependent protein kinase catalytic subunit
• UniProt Protein Name: DNA-dependent protein kinase catalytic subunit
• UniProt Gene Name: Prkdc
• UniProt Synonym Gene Names: Xrcc7; DNA-PK catalytic subunit; DNA-PKcs
• UniProt Entry Name: PRKDC_MOUSE