CD86 protein
CD86-muIg-Biotin
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
CD86; N/A; CD86-muIg-Biotin; Human CD86-muIg Fusion Protein; CD86 protein
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
323
Applicable Applications for CD86 protein
ELISA, FCM/FACS (Flow Cytometry)
Molecular Structure
A soluble fusion protein consisting of the extracellular (224aa) domain of human CD86 fused to murine IgG2a Fc (232aa). Predicted monomeric molecular weight 52.2 kd.
Transfectant Cell Line
CHO
Production
Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Performance
CD86-muIg/Biotin was highly reactive at 5 ng/ml in an Enzyme Immuno Assay utilizing immobilized anti-CD86 antibody (Catalog #MBS666200) for capture, followed by Streptavidin/HRP and TMB/H2O2 substrate chromagen for detection. It was also active at 50-100 ng/ml in a similar EIA utilizing immobilized recombinant CD152-muIg (Cat # MBS666792) for capture.
Purified CD86-muIg blocked binding of CD152-muIg to CD86 on Raji cells in FACS. Five x 105 Raji cell per tube were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD80 mAb (Catalog #MBS666674) so that only CD86 binding would be apparent. They were then incubated 45 minutes on ice with 80 ul of CD152-muIg/R-PE (Catalog# MBS666880) at a concentration of 0.2 ug/ml, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.83Log 10 fluorescent units when compared to a Mouse IgG2a isotype control (Catalog #MBS666441) at a similar concentration. Binding was 60% blocked when the CD152-muIg/R-PE reagent was pre incubated with 50 ug/ml of CD86-muIg.
Purified CD86-muIg blocked binding of CD152-muIg to CD86 on Raji cells in FACS. Five x 105 Raji cell per tube were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD80 mAb (Catalog #MBS666674) so that only CD86 binding would be apparent. They were then incubated 45 minutes on ice with 80 ul of CD152-muIg/R-PE (Catalog# MBS666880) at a concentration of 0.2 ug/ml, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.83Log 10 fluorescent units when compared to a Mouse IgG2a isotype control (Catalog #MBS666441) at a similar concentration. Binding was 60% blocked when the CD152-muIg/R-PE reagent was pre incubated with 50 ug/ml of CD86-muIg.
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Related Product Information for CD86 protein
Information: Human CD86 (B7-2) is a costimulating ligand for CD28 and CTLA-4. CD86 is expressed on activated B cells and blood monocytes(3).
References
1. C. Caux, et al, (1994) J Exp Med 180: 1841-1847. 2. C.B. Thompson, (1995) Cell 81: 979-982. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 703-705. 4. D. Mauri, et al, (1995) J Immunol 155: 118-127.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,420 Da
NCBI Official Full Name
CD86, partial
NCBI Official Synonym Full Names
CD86 molecule
NCBI Official Symbol
CD86
NCBI Official Synonym Symbols
B70; B7-2; B7.2; LAB72; CD28LG2
NCBI Protein Information
T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
UniProt Protein Name
T-lymphocyte activation antigen CD86
UniProt Gene Name
CD86
UniProt Synonym Gene Names
CD28LG2
UniProt Entry Name
CD86_HUMAN
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Product Notes
The CD86 cd86 (Catalog #AAA78191) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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