Application Data
(HTRF assay for FAK (409-698) activity 1 uM TK substrate was incubated with different concentrations of FAK protein and 100 uM ATP in the reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All the operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
FAK recombinant protein
Recombinant FAK (409-698) protein
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Synonyms
FAK; N/A; Recombinant FAK (409-698) protein; PTK2; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; FAK recombinant protein
Host
Baculovirus
Form/Format
Recombinant FAK (409-698) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. Kinase Activity Assay Conditions: 1uM TK substrate was incubated with different concentrations of FAK (409-698)protein in a 10ul reaction system containing 1X Enzymatic Buffer, 5mM MgCl2, 1mM DTT, 5nm SEB and 100uM ATP for1 hour. The 10ul detection reagents containing TK antibody and SA-XL665, each of which was 1:100 diluted with 1X Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for FAK (409-698) activity 1 uM TK substrate was incubated with different concentrations of FAK protein and 100 uM ATP in the reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All the operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for FAK (409-698) activity 1 uM TK substrate was incubated with different concentrations of FAK protein and 100 uM ATP in the reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All the operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
SDS-PAGE
(Recombinant FAK (409-698) protein gel 10% SDS-PAGE Coomassie staining MW: 34.8 kDa Purity: >90%)
SDS-PAGE
(Recombinant FAK (409-698) protein gel 10% SDS-PAGE Coomassie staining MW: 34.8 kDa Purity: >90%)
Related Product Information for FAK recombinant protein
Short Description: Recombinant FAK (409-698) protein includes amino acids 409-698 of human FAK protein (accession number NP_722560.1); it was expressed in a baculovirus expression system with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 34.8 kDa. This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: FAK (Focal Adhesion Kinase 1), also known as PTK2 (Protein Tyrosine Kinase 2), is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. It is required for early embryonic development and placenta development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation and normal heart development. FAK regulates axon growth and neuronal cell migration, axon branching and synapse formation, and it is required for normal development of the nervous system. It also plays a role in osteogenesis and differentiation of osteoblasts. FAK can function in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. It can form multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. FAK also can regulate numerous signaling pathways, promote activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. It can recruit the ubiquitin ligase MDM2 to p53/TP53 in the nucleus, and thereby regulates p53/TP53 activity, p53/TP53 ubiquitination and proteasomal degradation.
Background: FAK (Focal Adhesion Kinase 1), also known as PTK2 (Protein Tyrosine Kinase 2), is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. It is required for early embryonic development and placenta development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation and normal heart development. FAK regulates axon growth and neuronal cell migration, axon branching and synapse formation, and it is required for normal development of the nervous system. It also plays a role in osteogenesis and differentiation of osteoblasts. FAK can function in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. It can form multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. FAK also can regulate numerous signaling pathways, promote activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. It can recruit the ubiquitin ligase MDM2 to p53/TP53 in the nucleus, and thereby regulates p53/TP53 activity, p53/TP53 ubiquitination and proteasomal degradation.
Product Categories/Family for FAK recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,253 Da
NCBI Official Full Name
focal adhesion kinase 1 isoform a
NCBI Official Synonym Full Names
protein tyrosine kinase 2
NCBI Official Symbol
PTK2
NCBI Official Synonym Symbols
FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
NCBI Protein Information
focal adhesion kinase 1
UniProt Protein Name
Focal adhesion kinase 1
UniProt Gene Name
PTK2
UniProt Synonym Gene Names
FAK; FAK1; FADK 1; FRNK; PPP1R71
UniProt Entry Name
FAK1_HUMAN
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Product Notes
The FAK ptk2 (Catalog #AAA60153) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FAK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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