IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSPB1-S82 pAb (AAA36652). Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S82 pAb (AAA36652) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight.)
Rabbit HSPB1-S82 Antibody | anti-HSPB1 antibody
Phospho-HSPB1-S82 Polyclonal Antibody
Cross-Reactivity
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSPB1-S82 pAb (AAA36652). Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S82 pAb (AAA36652) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSPB1-S82 pAb (AAA36652). Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S82 pAb (AAA36652) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S82 pAb (AAA36652) at 1:1000 dilution or HSPB1 antibody . HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by Anisomycin (25 ug/mL) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S82 pAb (AAA36652) at 1:1000 dilution or HSPB1 antibody . HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by Anisomycin (25 ug/mL) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by EGF using 2.5ug Phospho-HSPB1-S82 antibody. Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S82 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by EGF using 2.5ug Phospho-HSPB1-S82 antibody. Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S82 antibody at a dilition of 1:1000.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Phospho-HSPB1-S82 antibody (AAA36652). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Phospho-HSPB1-S82 antibody (AAA36652). Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S82 antibody at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1min.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S82 antibody at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1min.)
NCBI and Uniprot Product Information
Calculated MW: 22 kDa
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Product Notes
The HSPB1 hspb1 (Catalog #AAA36652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSPB1-S82 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSPB1 hspb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPB1-S82, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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