ELISA
(ELISA on Denge Virus-Like Particles using AAA14182 Dengue Virus Serotype 2 VLPs were coated onto a plate at 5µg/ml. Anti-flavivirus antibody (AAA14182) added to plate in a 3-fold serial dilution starting at 1000 ng/ml. Detection performed using HRP labelled goat anti-mouse IgG.)
Flavivirus group antigen Recombinant Antibody
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]
Reactivity
Dengue Virus, Zika Virus, West Nile Virus, Flaviviridae, Yellow Fever Virus
Applications
Immunofluorescence, Western Blot, ELISA, Flow Cytometry, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
Flavivirus group antigen; N/A; Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]; Protein E; Envelope Protein; Flavivirus group antigen recombinant antibody
Host
Rabbit
Reactivity
Dengue Virus, Zika Virus, West Nile Virus, Flaviviridae, Yellow Fever Virus
Isotype
Rabbit IgG, kappa
Clone Number
D1-4G2-4-15 (4G2)
Specificity
Recognizes flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS only with 0.02% Proclin 300.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for Flavivirus group antigen recombinant antibody
Immunofluorescence (IF), Western Blot (WB), NTRL, ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC)
Application Notes
This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.
Immunogen
Dengue Virus type 2 antigens.
Preparation and Storage
Store at 4 degree C for up to 3 months . For longer storage, aliquot and store at -20 degree C.
ELISA
(ELISA on Denge Virus-Like Particles using AAA14182 Dengue Virus Serotype 2 VLPs were coated onto a plate at 5µg/ml. Anti-flavivirus antibody (AAA14182) added to plate in a 3-fold serial dilution starting at 1000 ng/ml. Detection performed using HRP labelled goat anti-mouse IgG.)
ELISA
(ELISA on Denge Virus-Like Particles using AAA14182 Dengue Virus Serotype 2 VLPs were coated onto a plate at 5µg/ml. Anti-flavivirus antibody (AAA14182) added to plate in a 3-fold serial dilution starting at 1000 ng/ml. Detection performed using HRP labelled goat anti-mouse IgG.)
IF (Immunofluorescence)
(Detection of Zika virus by immunofluorescence using AAA14182 Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as controls. Cells were fixed in 4% PFA for 30 min at RT. Primary antibody (AAA14182, rabbit IgG) staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4°C, detected using a Goat Anti-Rabbit IgG (AF568, 1:500) for 1h at RT and counterstained with DAPI.)
IF (Immunofluorescence)
(Detection of Zika virus by immunofluorescence using AAA14182 Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as controls. Cells were fixed in 4% PFA for 30 min at RT. Primary antibody (AAA14182, rabbit IgG) staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4°C, detected using a Goat Anti-Rabbit IgG (AF568, 1:500) for 1h at RT and counterstained with DAPI.)
Related Product Information for Flavivirus group antigen recombinant antibody
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for Flavivirus group antigen recombinant antibody
References
Nawa et al. Development of dengue IgM-capture enzyme-linked immunosorbent assay with higher sensitivity using monoclonal detection antibody. J Virol Methods PMID:11164919
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Product Notes
The Flavivirus group antigen (Catalog #AAA14182) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] reacts with Dengue Virus, Zika Virus, West Nile Virus, Flaviviridae, Yellow Fever Virus and may cross-react with other species as described in the data sheet. AAA Biotech's Flavivirus group antigen can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), NTRL, ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC). This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection. Researchers should empirically determine the suitability of the Flavivirus group antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flavivirus group antigen, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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