Rabbit anti-Human 4-1BB Antibody | anti-4-1BB antibody

Anti-4-1BB [BBK4 ], Rabbit IgG

Cat. Num. AAA72285

• Gene Names: TNFRSF9; ILA; 4-1BB; CD137; CDw137
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A affinity purified
• Synonyms: 4-1BB; N/A; Anti-4-1BB [BBK4 ], Rabbit IgG; Anti-4-1BB [BBK4 ]; CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9; TNFRSF9; T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; BBK-4; anti-4-1BB antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: Rabbit IgG, Kappa
• Clone Number: BBK4
• Specificity: This antibody is specific for human CD137.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-4-1BB antibody: FCM/FACS (Flow Cytometry), ELISA, Antagonistic Antibody
• Immunogen: The original antibody was raised by immunizing a mouse with CD137.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

IF (Immunofluorescence)

(Immunofluorescence staining of human peripheral blood leukocytes with anti-4-1BB (AAA72285) BBK4 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes stained with the chimeric rabbit IgG version of BBK4 (AAA72285-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72285-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)

WB (Western Blot)

(Western Blot using anti-4-1BB antibody BBK4 (AAA72285). Human peripheral blood monocytes(A) (0.3ug/ml) cells lysate and human tonsil(B) (1ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of BBK4 (AAA72285-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

Related Product Information for anti-4-1BB antibody

This antibody stimulates T-cell activity (Son et al, 2004; pmid:15087232). ELISA was performed using this antibody on E.coli expressed human 4-1BB (Son et al, 2004; pmid:15087232). Mixed lymphocyte reaction was investigated with fresh peripheral blood mononuclear cells using this antibody (Son et al, 2004; pmid:15087232). This antibody was used for flow cytometry on a subline of human Jurkat 8-1 cells (Son et al, 2004; pmid:15087232). This antibody promoted long-term proliferation of PBMC (Son et al, 2004; pmid:15087232).

Product Categories/Family for anti-4-1BB antibody

immunology; cancer; oncology

References

Son et al. Humanization of agonistic anti-human 4-1BB monoclonal antibody using a phage-displayed combinatorial library. J Immunol Methods. 2004 Mar;286(1-2):187-201. PMID:15087232

NCBI and Uniprot Product Information

• NCBI GI #: 5730095
• NCBI GeneID: 3604
• NCBI Accession #: NP_001552.2
• NCBI GenBank Nucleotide #: NM_001561.5
• UniProt Accession #: Q07011; AAB34924
• Molecular Weight: 27,899 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 9
• NCBI Official Symbol: TNFRSF9
• NCBI Official Synonym Symbols: ILA; 4-1BB; CD137; CDw137
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interl
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 9
• UniProt Gene Name: TNFRSF9
• UniProt Synonym Gene Names: CD137; ILA
• UniProt Entry Name: TNR9_HUMAN