Application Data
Rabbit FAK (pY576) Antibody | anti-FAK (pY576) antibody
Rabbit anti Phospho-FAK (pTyr 576)
Reactivity
Human; Rat; Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Synonyms
FAK (pY576); Rabbit anti Phospho-FAK (pTyr 576); Rabbit anti-Phospho-FAK (pY576); Focal Adhesion Kinase (FAK); FAK1; FADK; pp125FAK; PTK2.; anti-FAK (pY576) antibody
Host
Rabbit
Reactivity
Human; Rat; Mouse
Specificity
This antibody recognizes FAK (pY576) with a phosphorylated site at Tyrosine 576. It does not crossreact with non-phosphospecific peptide.
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-FAK (pY576) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100 tested
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100 tested
Volume
0.2ml
Antigen Preparation
A synthetic peptide surrounding to the epitope -STYYKA- with a phosphorylation site at Tyrosine 576 of human FAK protein. This sequence is identical among human, mouse and rat.
Positive Control
Conditioned 3T3 cell lysate
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Application Data
Application Data
Related Product Information for anti-FAK (pY576) antibody
Focal adhesion kinase (FAK) gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinase but lacks significant sequence similarity to kinase from other subfamilies. Activation by phosphorylation of Y397, Y576/577 and Y863 of FAK protein may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation
References
Leu TH, et al. Tyr-863 phosphorylation enhances focal adhesion kinase autophosphorylation at Tyr-397. Oncogene. 2002 Oct 10;21 (46):6992-7000.
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Product Notes
The FAK (pY576) (Catalog #AAA14094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho-FAK (pTyr 576) reacts with Human; Rat; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAK (pY576) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100 tested. Researchers should empirically determine the suitability of the FAK (pY576) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK (pY576), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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