WB (Western Blot)
(Western Blot using anti-polysialic acid antibody Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with at 2 µg/ml before being detected by a secondary antibody.)
Polysialic acid Recombinant Antibody
Anti-Polysialic acid [735]
Reactivity
Human, Mouse, Rat
Applications
Immunoblot, Western Blot, Immunoprecipitation, Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
Polysialic acid; N/A; Anti-Polysialic acid [735]; PSA; N-CAM; neural cell adhesion molecule; polySA; Polysialic acid recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG, kappa
Clone Number
735
Specificity
Recognizes Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1 mg/mL (varies by lot)
Sequence Length
346
Applicable Applications for Polysialic acid recombinant antibody
Immunoblot, Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC), Immunohistochemistry (IHC)
Application Notes
Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).
Immunogen
Homopolymers of α2, 8-linked sialic acid of Escherichia coli K1.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
WB (Western Blot)
(Western Blot using anti-polysialic acid antibody Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with at 2 µg/ml before being detected by a secondary antibody.)
WB (Western Blot)
(Western Blot using anti-polysialic acid antibody Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with at 2 µg/ml before being detected by a secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of rat spinal chord using anti-polysialic acid antibody AAA14183 Formalin fixed rat spinal chord slices were were stained with a AAA14183 at 3 µg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of rat spinal chord using anti-polysialic acid antibody AAA14183 Formalin fixed rat spinal chord slices were were stained with a AAA14183 at 3 µg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of guinea pig brain using anti-polysialic acid antibody AAA14183 Formalin fixed guinea pig brain slices were were stained with AAA14183 at 3 µg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of guinea pig brain using anti-polysialic acid antibody AAA14183 Formalin fixed guinea pig brain slices were were stained with AAA14183 at 3 µg/ml.)
Related Product Information for Polysialic acid recombinant antibody
Recombinant monoclonal antibody to Polysialic acid. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 735.
Product Categories/Family for Polysialic acid recombinant antibody
References
Bitter-Suermann & Roth EMonoclonal antibodies to polysialic acid reveal epitope sharing between invasive pathogenic bacteria, differentiating cells and tumor cells. Immunol Res. 1987; 6(4):225-37. PMID:2448401
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
polysialic acid capsule biosynthesis sialic acid synthase NeuB
Similar Products
Product Notes
The Polysialic acid (Catalog #AAA14183) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Polysialic acid [735] reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Polysialic acid can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC), Immunohistochemistry (IHC). Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006). Researchers should empirically determine the suitability of the Polysialic acid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Polysialic acid, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.