Rabbit Cytokeratin Antibody | anti-KRT76 antibody

Cytokeratin, Basic (Type II or HMW)

Cat. Num. AAA215349

• Gene Names: KRT76; KRT2B; KRT2P; HUMCYT2A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cytokeratin; N/A; Cytokeratin, Basic (Type II or HMW); KRT2B; KRT2P; HUMCYT2A; Keratin; type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9; anti-KRT76 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Clone Number: KRTH/2147R
• Specificity: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 638

• Applicable Applications for anti-KRT76 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.
• Immunogen: Solubilized keratin extract from human stratum corneum
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 12q13.13
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with CK HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)

SDS-PAGE

(SDS-PAGE Analysis of Purified CK HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CK HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R).)

References

1. Varma M et. al. Mod Pathol 1999;12(5):472-8. 2. Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58

NCBI and Uniprot Product Information

• NCBI GI #: 153791670
• NCBI GeneID: 51350
• NCBI Accession #: NP_056932.2
• NCBI GenBank Nucleotide #: NP_056932.2
• UniProt Accession #: Q01546
• Molecular Weight: 50-67kDa
• NCBI Official Full Name: keratin, type II cytoskeletal 2 oral
• NCBI Official Synonym Full Names: keratin 76
• NCBI Official Symbol: KRT76
• NCBI Official Synonym Symbols: KRT2B; KRT2P; HUMCYT2A
• NCBI Protein Information: keratin, type II cytoskeletal 2 oral
• UniProt Protein Name: Keratin, type II cytoskeletal 2 oral
• UniProt Gene Name: KRT76
• UniProt Synonym Gene Names: KRT2B; KRT2P; CK-2P; K2P; K76
• UniProt Entry Name: K22O_HUMAN