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product-image-AAA214635_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis Purified Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)

Rabbit Cytokeratin, Basic Monoclonal Antibody | anti-KRT76 antibody

Cytokeratin, Basic (Type II or HMW) (Epithelial Marker)

Gene Names
KRT76; KRT2B; KRT2P; HUMCYT2A
Reactivity
Human. Expected to show broad species reactivity.
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Cytokeratin, Basic, Antibody; Cytokeratin, Basic (Type II or HMW) (Epithelial Marker); KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9; anti-KRT76 antibody
Ordering
Host
Rabbit
Reactivity
Human. Expected to show broad species reactivity.
Clonality
Monoclonal
Isotype
IgG
Clone Number
KRTH/1576R
Specificity
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor.
Form/Format
200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
638
Applicable Applications for anti-KRT76 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant full-length human KRT76 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
12q13.13
Positive Control
Epithelial cells, Skin or Adenocarcinomas
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)

product-image-AAA214635_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis Purified Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)

product-image-AAA214635_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)

product-image-AAA214635_IHC15.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/1576R).)
References
Tseng SCG et. al. Cell 1982; 30361. Woodock-Mitchell J et. al. Journal of Cell Biology 1982; 95:580-8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52-67kDa
NCBI Official Full Name
keratin, type II cytoskeletal 2 oral
NCBI Official Synonym Full Names
keratin 76
NCBI Official Symbol
KRT76
NCBI Official Synonym Symbols
KRT2B; KRT2P; HUMCYT2A
NCBI Protein Information
keratin, type II cytoskeletal 2 oral
UniProt Protein Name
Keratin, type II cytoskeletal 2 oral
UniProt Gene Name
KRT76
UniProt Synonym Gene Names
KRT2B; KRT2P; CK-2P; K2P; K76

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Product Notes

The KRT76 krt76 (Catalog #AAA214635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) reacts with Human. Expected to show broad species reactivity. and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin, Basic can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the KRT76 krt76 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin, Basic, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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