IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin Recombinant Rabbit Monoclonal Antibody (UBB/3143R).)
Rabbit anti-Human Ubiquitin Antibody | anti-UBB antibody
Ubiquitin (Autophagy Marker)
Gene Names
UBB; HEL-S-50
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Ubiquitin; N/A; Ubiquitin (Autophagy Marker); Epididymis secretory protein Li 50; Polyubiquitin B; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; anti-UBB antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Clone Number
UBB/3143R
Specificity
Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-UBB antibody
IHC (Immunohistochemistry)
Positive Control
HeLa or Jurkat cells. Alzheimer's Brain.
Immunogen
Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
Cellular Localization
Cell Surface, Cytoplasmic and Nuclear
Hu-chromosome Location
17p11.2
Antibody Type
Recombinant Rabbit Monoclonal Antibody
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin Recombinant Rabbit Monoclonal Antibody (UBB/3143R).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin Recombinant Rabbit Monoclonal Antibody (UBB/3143R).)
SDS-PAGE
(SDS-PAGE Analysis Purified Ubiquitin Recombinant Rabbit Monoclonal Antibody (UBB/3143R). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Ubiquitin Recombinant Rabbit Monoclonal Antibody (UBB/3143R). Confirmation of Purity and Integrity of Antibody.)
References
Hochstrasser, M. 1995. Curr. Opin. Cell Biol. 7: 215-223.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The UBB (Catalog #AAA215247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin (Autophagy Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the UBB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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