Rat CD52 Antibody | anti-CD52 antibody

Anti-CD52 [Campath-1H], Rat IgG2b

Cat. Num. AAA14187

• Gene Names: CD52; CDW52; EDDM5
• Reactivity: Human, Rhesus Monkey, Cynomolgus Monkey
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Protein A affinity purified
• Synonyms: CD52; Anti-CD52 [Campath-1H]; Rat IgG2b; CDW52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; YTH66.9HL; Alemtuzumab; anti-CD52 antibody

• Host: Rat
• Reactivity: Human, Rhesus Monkey, Cynomolgus Monkey
• Isotype: Rat IgG2b, Kappa
• Clone Number: Campath-1H
• Specificity: Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD52 antibody: Cytotoxicity (CT), ELISA (EIA), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), in vivo, Flow Cytometry (FC/FACS), Western Blot (WB)
• Immunogen: Human lymphocytes.
• Origin Pub PMID: 10366506
• Chimeric Use Statement: This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

IHC (Immunohistochemistry)

(Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody AAA14187 Formalin fixed human thyroid cancer slices were were stained with a AAA14187-10.0 at 5 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescent staining of rat thymus using anti-CD52 antibody AAA14187 Formaldehyde-fixed rat thymus slices were stained with AAA14187-10.0 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. AAA14187 shows cross-reactivity with rat.)

FCM (Flow Cytometry)

(Flow-cytometry using anti-CD52 antibody Campath-1H (AAA14187) Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Campath-1H at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

FCM (Flow Cytometry)

(Flow-cytometry using anti-CD52 antibody Campath-1H Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Campath-1H at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat thymus tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat spleen tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat epididymis tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H (AAA14187) Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (AAA14187) at 5 ug/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; AAA14187 (FITC), DAPI and merged channels.)

Product Categories/Family for anti-CD52 antibody

biosimilar; biotherapeuticresearch

References

Riechmann L, Clark M, Waldmann H, Winter G. Reshaping human antibodies for therapy. Nature. 1988 Mar 24;332(6162):323-7. PMID:3127726

NCBI and Uniprot Product Information

• NCBI GI #: 399214
• NCBI GeneID: 1043
• UniProt Accession #: P31358; Q5T138; Q9BW46
• Molecular Weight: 6,614 Da
• NCBI Official Full Name: CAMPATH-1 antigen
• NCBI Official Synonym Full Names: CD52 molecule
• NCBI Official Symbol: CD52
• NCBI Official Synonym Symbols: CDW52; EDDM5
• NCBI Protein Information: CAMPATH-1 antigen
• UniProt Protein Name: CAMPATH-1 antigen
• UniProt Gene Name: CD52
• UniProt Synonym Gene Names: CDW52; HE5; He5