Standard Curve (Sample)
(Typical Standard Curve for ACSL4 ELISA (Sandwich))
Rat Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) ELISA Kit | ACSL4 elisa kit
Rat Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) ELISA Kit
Gene Names
Acsl4; Acs4; Facl4
Reactivity
Rat
Synonyms
Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4); N/A; Rat Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) ELISA Kit; ACSL4 ELISA kit (Rat); ACS4; FACL4; LACS4; MRX63; MRX68; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Mental Retardation, X-Linked 63; Lignoceroyl-CoA Synthase; ACSL4 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4). No significant cross-reactivity or interference between Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) and analogues was observed.
Reactive with Rat ACSL4/Acyl Coenzyme A Synthetase Long Chain Family, Member 4
Reactive with Rat ACSL4/Acyl Coenzyme A Synthetase Long Chain Family, Member 4
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Method
Colormetric
Featured Series
SE kit
Detection Range
0.312-20ng/mL
Sensitivity
0.131ng/mL
Sample Size
100uL
Assay Time
3h
Function
Detects protein (regular version)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Enzyme & Kinase
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
(Typical Standard Curve for ACSL4 ELISA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for ACSL4 ELISA (Sandwich))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,327 Da
NCBI Official Full Name
long-chain-fatty-acid--CoA ligase 4
NCBI Official Synonym Full Names
acyl-CoA synthetase long-chain family member 4
NCBI Official Symbol
Acsl4
NCBI Official Synonym Symbols
Acs4; Facl4
NCBI Protein Information
long-chain-fatty-acid--CoA ligase 4; LACS 4; long-chain acyl-CoA synthetase 4; fatty acid-Coenzyme A ligase long chain 4; fatty acid Coenzyme A ligase, long chain 4; fatty acid-Coenzyme A ligase, long chain 4
UniProt Protein Name
Long-chain-fatty-acid--CoA ligase 4
UniProt Gene Name
Acsl4
UniProt Synonym Gene Names
Acs4; Facl4; LACS 4
UniProt Entry Name
ACSL4_RAT
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Product Notes
The Rat ACSL4 acsl4 (Catalog #AAA161497) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161497 ELISA Kit recognizes Rat ACSL4. It is sometimes possible for the material contained within the vial of "Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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