Standard Curve (Sample)
Rat ATP synthase subunit e, mitochondrial (ATP5I) ELISA Kit | ATP5I elisa kit
Rat ATP synthase subunit e, mitochondrial (ATP5I) ELISA Kit
Gene Names
ATP5I; ATP5K
Reactivity
Rat
Synonyms
ATP synthase subunit e, mitochondrial (ATP5I); N/A; Rat ATP synthase subunit e, mitochondrial (ATP5I) ELISA Kit; ATP5I elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ATP5I elisa kit
Intended Uses: This ATP5I ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ATP5I. This ELISA kit for research use only!
Principle of the Assay: ATP5I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP5I antibody and an ATP5I-HRP conjugate. The assay sample and buffer are incubated together with ATP5I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP5I concentration since ATP5I from samples and ATP5I-HRP conjugate compete for the anti-ATP5I antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP5I from the sample, fewer sites are left to bind ATP5I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP5I concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ATP5I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP5I antibody and an ATP5I-HRP conjugate. The assay sample and buffer are incubated together with ATP5I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP5I concentration since ATP5I from samples and ATP5I-HRP conjugate compete for the anti-ATP5I antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP5I from the sample, fewer sites are left to bind ATP5I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP5I concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ATP5I elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,933 Da
NCBI Official Full Name
ATP synthase subunit e, mitochondrial
NCBI Official Synonym Full Names
ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E
NCBI Official Symbol
ATP5I
NCBI Official Synonym Symbols
ATP5K
NCBI Protein Information
ATP synthase subunit e, mitochondrial; ATPase subunit e; ATP synthase e chain, mitochondrial; F1F0-ATP synthase, murine e subunit; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
UniProt Protein Name
ATP synthase subunit e, mitochondrial
UniProt Gene Name
ATP5I
UniProt Synonym Gene Names
ATP5K; ATPase subunit e
UniProt Entry Name
ATP5I_HUMAN
Similar Products
Product Notes
The Rat ATP5I atp5i (Catalog #AAA244772) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244772 ELISA Kit recognizes Rat ATP5I. It is sometimes possible for the material contained within the vial of "ATP synthase subunit e, mitochondrial (ATP5I), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.