Reactivity
Rat
Specificity
No cross-reaction with other factors.
Sequence Length
482
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for C3a elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat C3a monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,864 Da
NCBI Official Full Name
C3a anaphylatoxin chemotactic receptor
NCBI Official Synonym Full Names
complement component 3a receptor 1
NCBI Official Symbol
C3AR1
NCBI Official Synonym Symbols
AZ3B; C3AR; HNFAG09
NCBI Protein Information
C3a anaphylatoxin chemotactic receptor; C3a-R; complement component 3 receptor 1
UniProt Protein Name
C3a anaphylatoxin chemotactic receptor
UniProt Gene Name
C3AR1
UniProt Synonym Gene Names
AZ3B; C3R1; HNFAG09; C3AR; C3a-R
UniProt Entry Name
C3AR_HUMAN