Standard Curve (Sample)
Rat Cytochrome P450 2B6 (CYP2B6) ELISA Kit | CYP2B6 elisa kit
Rat Cytochrome P450 2B6 (CYP2B6) ELISA Kit
Gene Names
CYP2B6; CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
Reactivity
Rat
Synonyms
Cytochrome P450 2B6 (CYP2B6); N/A; Rat Cytochrome P450 2B6 (CYP2B6) ELISA Kit; CYP2B6 elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CYP2B6 elisa kit
Intended Uses: This CYP2B6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CYP2B6. This ELISA kit for research use only!
Principle of the Assay||CYP2B6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYP2B6 antibody and an CYP2B6-HRP conjugate. The assay sample and buffer are incubated together with CYP2B6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2B6 concentration since CYP2B6 from samples and CYP2B6-HRP conjugate compete for the anti-CYP2B6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2B6 from the sample, fewer sites are left to bind CYP2B6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2B6 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||CYP2B6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYP2B6 antibody and an CYP2B6-HRP conjugate. The assay sample and buffer are incubated together with CYP2B6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2B6 concentration since CYP2B6 from samples and CYP2B6-HRP conjugate compete for the anti-CYP2B6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2B6 from the sample, fewer sites are left to bind CYP2B6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2B6 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,385 Da
NCBI Official Full Name
cytochrome P450 2B6
NCBI Official Synonym Full Names
cytochrome P450, family 2, subfamily B, polypeptide 6
NCBI Official Symbol
CYP2B6
NCBI Official Synonym Symbols
CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
NCBI Protein Information
cytochrome P450 2B6; cytochrome P450 IIB1; 1,4-cineole 2-exo-monooxygenase; cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6
UniProt Protein Name
Cytochrome P450 2B6
UniProt Gene Name
CYP2B6
UniProt Entry Name
CP2B6_HUMAN
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Product Notes
The Rat CYP2B6 cyp2b6 (Catalog #AAA244711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244711 ELISA Kit recognizes Rat CYP2B6. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2B6 (CYP2B6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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