Standard Curve (Sample)
Rat Cytoplasmic tyrosine-protein kinase BMX (BMX) ELISA Kit | BMX elisa kit
Rat Cytoplasmic tyrosine-protein kinase BMX (BMX) ELISA Kit
Gene Names
BMX; ETK; PSCTK2; PSCTK3
Reactivity
Rat
Synonyms
Cytoplasmic tyrosine-protein kinase BMX (BMX); N/A; Rat Cytoplasmic tyrosine-protein kinase BMX (BMX) ELISA Kit; BMX elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for BMX elisa kit
Intended Uses: This BMX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BMX. This ELISA kit for research use only!
Principle of the Assay: BMX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMX antibody and an BMX-HRP conjugate. The assay sample and buffer are incubated together with BMX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMX concentration since BMX from samples and BMX-HRP conjugate compete for the anti-BMX antibody binding site. Since the number of sites is limited, as more sites are occupied by BMX from the sample, fewer sites are left to bind BMX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMX concentration in each sample is interpolated from this standard curve.
Principle of the Assay: BMX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMX antibody and an BMX-HRP conjugate. The assay sample and buffer are incubated together with BMX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMX concentration since BMX from samples and BMX-HRP conjugate compete for the anti-BMX antibody binding site. Since the number of sites is limited, as more sites are occupied by BMX from the sample, fewer sites are left to bind BMX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMX concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BMX elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,011 Da
NCBI Official Full Name
cytoplasmic tyrosine-protein kinase BMX
NCBI Official Synonym Full Names
BMX non-receptor tyrosine kinase
NCBI Official Symbol
BMX
NCBI Official Synonym Symbols
ETK; PSCTK2; PSCTK3
NCBI Protein Information
cytoplasmic tyrosine-protein kinase BMX; NTK38 tyrosine kinase; BTK-like on X chromosome; Etk/Bmx cytosolic tyrosine kinase; epithelial and endothelial tyrosine kinase; bone marrow tyrosine kinase gene in chromosome X protein
UniProt Protein Name
Cytoplasmic tyrosine-protein kinase BMX
UniProt Gene Name
BMX
UniProt Synonym Gene Names
ETK
UniProt Entry Name
BMX_HUMAN
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Product Notes
The Rat BMX bmx (Catalog #AAA245241) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245241 ELISA Kit recognizes Rat BMX. It is sometimes possible for the material contained within the vial of "Cytoplasmic tyrosine-protein kinase BMX (BMX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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