Rat E-Cadherin PicoKine ELISA Kit | Cdh1 elisa kit

Rat E-Cadherin PicoKine ELISA Kit

Cat. Num. AAA46191

• Reactivity: Rat
• Synonyms: E-Cadherin PicoKine; N/A; Rat E-Cadherin PicoKine ELISA Kit; Cadherin-1; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; cadherin 1, type 1, E-cadherin (epithelial); Cdh1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat E-Cadherin
• Sequence Length: 886
• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Sample: For quantitative detection of rat E-Cadherin in cell culture supernates, serum and plasma(heparin).

• Immunogen: NSO, Q24-S704
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each rat E-Cadherin standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1571|4158|7193|1690|4873|8056; Standard deviation|75.41|228.69|431.58|119.99|302.13|459.19; CV(%)|4.8|5.5|6.0|7.1|6.2|5.7
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.097|0.100|0.163|0.252|0.482|0.930|1.654|2.438
• Anticoagulant: Heparin
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Rat E-Cadherin PicoKine ELISA Kit standard curve)

Related Product Information for Cdh1 elisa kit

Principle of the assay: Rat E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Cadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q24-S704) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat E-Cadherin amount of sample captured in plate.

Background: Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 13786164
• NCBI GeneID: 83502
• NCBI Accession #: NP_112624.1
• NCBI GenBank Nucleotide #: NM_031334.1
• UniProt Accession #: Q9R0T4; O35794; Q9JIV9
• Molecular Weight: 98,715 Da
• NCBI Official Full Name: cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_RAT