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product-image-AAA129525_SC15.jpg Standard Curve (Sample)

Rat Fatty Acid Transport Protein 1 (FATP1) ELISA Kit | FATP1 elisa kit

Rat Fatty Acid Transport Protein 1 (FATP1) ELISA Kit

Reactivity
Rat
Synonyms
Fatty Acid Transport Protein 1 (FATP1); N/A; Rat Fatty Acid Transport Protein 1 (FATP1) ELISA Kit; SLC27A1; ACSVL5; FATP; Long-Chain Fatty Acid Transport Protein 1; Solute Carrier Family 27 Member 1,Fatty Acid Transporter; FATP1 elisa kit
Ordering
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Fatty Acid Transport Protein 1 (FATP1).
No significant cross-reactivity or interference between Fatty Acid Transport Protein 1 (FATP1) and analogues was observed.
Sequence Length
646
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.058ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Fatty Acid Transport Protein 1 (FATP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Fatty Acid Transport Protein 1 (FATP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

product-image-AAA129525_SC15.jpg Standard Curve (Sample)
Related Product Information for FATP1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Transport Protein 1 (FATP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Transport Protein 1 (FATP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Transport Protein 1 (FATP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Transport Protein 1 (FATP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FATP1 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
72,599 Da
NCBI Official Full Name
fatty acid transport protein-1
NCBI Official Synonym Full Names
solute carrier family 27 (fatty acid transporter), member 1
NCBI Official Symbol
SLC27A1
NCBI Protein Information
long-chain fatty acid transport protein 1
UniProt Protein Name
Fatty acid transport protein-1
UniProt Entry Name
Q2L7E6_CHICK

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Product Notes

The Rat FATP1 (Catalog #AAA129525) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA129525 ELISA Kit recognizes Rat FATP1. It is sometimes possible for the material contained within the vial of "Fatty Acid Transport Protein 1 (FATP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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