Reactivity
Rat
Specificity
This kit recognizes Rat GDF11 in samples. No significant cross-reactivity or interference between Rat GDF11 and analogues was observed.
Sequence Length
407
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.63-1000pg/mL
Sensitivity
9.38pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GDF11 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GDF11 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Related Product Information for GDF11 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GDF11 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GDF11. You can calculate the concentration of Rat GDF11 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GDF11. You can calculate the concentration of Rat GDF11 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,091 Da
NCBI Official Full Name
growth/differentiation factor 11
NCBI Official Synonym Full Names
growth differentiation factor 11
NCBI Official Symbol
GDF11
NCBI Official Synonym Symbols
BMP11; BMP-11
NCBI Protein Information
growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11
UniProt Protein Name
Growth/differentiation factor 11
UniProt Gene Name
GDF11
UniProt Synonym Gene Names
BMP11; GDF-11; BMP-11
UniProt Entry Name
GDF11_HUMAN