Rat IL-10 ELISA Kit | IL-10 elisa kit

Rat IL-10 PicoKine Quick ELISA Kit

Cat. Num. AAA128053

• Gene Names: Il10; IL10X
• Reactivity: Rat
• Applications: ELISA
• Synonyms: IL-10; N/A; Rat IL-10 PicoKine Quick ELISA Kit; Interleukin-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10; IL-10 elisa kit

• Reactivity: Rat
• Samples: cell culture supernatants, serum and plasma (EDTA)
• Assay Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <4pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: S19-N178
• Anticoagulant: EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for IL-10 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.059	0.168	0.259	0.466	0.893	1.550	1.843	2.039

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	55	213	839	51	222	910
  Standard deviation	4.34	9.58	36.07	4.74	12.21	39.13
  CV (%)	7.9%	4.5%	4.3%	9.3%	5.5%	4.3%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	55	58	57	49	54	3.49	6.4%
  Sample 2	213	219	232	225	222	7.04	3.1%
  Sample 3	839	901	925	826	872	41.39	4.7%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Rat IL-10 PicoKine ELISA Kit standard curve)

Related Product Information for IL-10 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for IL-10 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 148747382
• NCBI GeneID: 25325
• NCBI Accession #: NP_036986.2
• NCBI GenBank Nucleotide #: NM_012854.2
• UniProt Accession #: P29456; Q63263
• Molecular Weight: 20,426 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: Il10
• NCBI Official Synonym Symbols: IL10X
• NCBI Protein Information: interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: Il10
• UniProt Synonym Gene Names: Il-10; IL-10; CSIF
• UniProt Entry Name: IL10_RAT