Standard Curve (Sample)
Rat MCP-1/CCL2 ELISA Kit | CCL2 elisa kit
Rat MCP-1/CCL2 PicoKine ELISA Kit
Gene Names
Ccl2; MCP-1; Scya2; Sigje
Reactivity
Rat
Synonyms
MCP-1/CCL2; N/A; Rat MCP-1/CCL2 PicoKine ELISA Kit; C-C motif chemokine 2; chemokine (C-C motif) ligand 2; CCL2; CCL2_HUMAN; Chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); Small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2; SMC-CF; CCL2 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Ccl2
Sequence Length
148
Samples
Cell culture supernatants and serum
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CCL2 elisa kit
Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3
Principle of the Assay: The Picokine™ Rat Ccl2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Ccl2 with a 96-well strip plate that is pre-coated with antibody specific for Ccl2. The detection antibody is a biotinylated antibody specific for Ccl2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ccl2 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q24-N148. The kit is analytically validated with ready-to-use reagents. To measure Rat Ccl2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Ccl2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl2 in the sample.
Principle of the Assay: The Picokine™ Rat Ccl2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Ccl2 with a 96-well strip plate that is pre-coated with antibody specific for Ccl2. The detection antibody is a biotinylated antibody specific for Ccl2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ccl2 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q24-N148. The kit is analytically validated with ready-to-use reagents. To measure Rat Ccl2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Ccl2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl2 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,460 Da
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
Ccl2
NCBI Official Synonym Symbols
MCP-1; Scya2; Sigje
NCBI Protein Information
C-C motif chemokine 2; small inducible gene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
Ccl2
UniProt Synonym Gene Names
Je; Mcp1; Scya2; MCP-1
UniProt Entry Name
CCL2_RAT
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Product Notes
The Rat CCL2 ccl2 (Catalog #AAA11589) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11589 ELISA Kit recognizes Rat CCL2. It is sometimes possible for the material contained within the vial of "MCP-1/CCL2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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