IF (Immunofluorescence)
(Immunofluorescence staining of CCL2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCL2 polyclonal antibody (dilution ratio 1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Golgi apparatus.)
Rabbit anti-Human MCP-1/CCL2 Polyclonal Antibody | anti-CCL2 antibody
Anti-MCP-1/CCL2 Antibody, Rabbit Polyclonal
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
MCP-1/CCL2, Antibody; Anti-MCP-1/CCL2 Antibody, Rabbit Polyclonal; MCP-1/CCL2 Antibody, Rabbit PAb, Antigen Affinity Purified; chemokine (C-C motif) ligand 2; Anti-GDCF-2 Antibody; Anti-HC11 Antibody; Anti-HSMCR30 Antibody; Anti-MCAF Antibody; Anti-MCP-1 Antibody; Anti-MCP1 Antibody; Anti-SCYA2 Antibody; Anti-SMC-CF Antibody; anti-CCL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MCP-1/CCL2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CCL2 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human MCP-1/CCL2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human MCP-1/CCL2 (rh MCP-1/CCL2). MCP-1/CCL2 specific IgG was purified by Human MCP-1/CCL2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of CCL2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCL2 polyclonal antibody (dilution ratio 1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Golgi apparatus.)
IF (Immunofluorescence)
(Immunofluorescence staining of CCL2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCL2 polyclonal antibody (dilution ratio 1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Golgi apparatus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CCL2 in human adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CCL2 in human adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-CCL2 antibody
Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
Product Categories/Family for anti-CCL2 antibody
References
Vasquez-Loarte T, Trubnykova M, Guio H. Genetic association meta-analysis: a new classification to assess ethnicity using the association of MCP-1 -2518 polymorphism and tuberculosis susceptibility as a model. BMC Genetics. 2015;16:128.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11kDa
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
CCL2
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1
UniProt Entry Name
CCL2_HUMAN
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Product Notes
The CCL2 ccl2 (Catalog #AAA254541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCP-1/CCL2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCP-1/CCL2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CCL2 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-1/CCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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