Standard Curve (Sample)
Rat Methylcytosine Dioxygenase TET3 (TET3) ELISA Kit | TET3 elisa kit
Rat Methylcytosine Dioxygenase TET3 (TET3) ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
Methylcytosine Dioxygenase TET3 (TET3); N/A; Rat Methylcytosine Dioxygenase TET3 (TET3) ELISA Kit; TET3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of TET3. No significant cross-reactivity or interference between TET3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TET3 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Competitive
Sensitivity
1.0 pg/mL
Applicable Applications for TET3 elisa kit
ELISA
Preparation and Storage
Storage: store at 2-8 degree C
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TET3 elisa kit
INTENDED USE: This TET3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TET3. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY: TET3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TET3 antibody and an TET3-HRP conjugate. The assay sample and buffer are incubated together with TET3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TET3 concentration since TET3 from samples and TET3-HRP conjugate compete for the anti-TET3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TET3 from the sample, fewer sites are left to bind TET3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TET3 concentration in each sample is interpolated from this standard curve.
PRINCIPLE OF THE ASSAY: TET3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TET3 antibody and an TET3-HRP conjugate. The assay sample and buffer are incubated together with TET3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TET3 concentration since TET3 from samples and TET3-HRP conjugate compete for the anti-TET3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TET3 from the sample, fewer sites are left to bind TET3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TET3 concentration in each sample is interpolated from this standard curve.
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Product Notes
The Rat TET3 (Catalog #AAA247933) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA247933 ELISA Kit recognizes Rat TET3. AAA Biotech's Methylcytosine Dioxygenase TET3 (TET3) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TET3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Methylcytosine Dioxygenase TET3 (TET3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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