DB (Dot Blot)
(Dot Blot analysis for recombinant TET3 (824-1795) protein. 250 ng of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 ug, 312.5 and 625 ng TET3 protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at room temperature for 1 hour. Sample was concentrated to 5 ul, then 1 ul sample was spotted onto positively charged nylon membrane. 5-hmC antibody was used to detect the generation of products.)
Tet3 recombinant protein
Recombinant Tet3 (824-1795) protein
Gene Names
TET3; hCG_40738
Synonyms
Tet3; N/A; Recombinant Tet3 (824-1795) protein; hCG_40738; Tet3 recombinant protein
Host
Baculovirus
Form/Format
Recombinant TET3 (824-1795) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% Glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
DB (Dot Blot)
(Dot Blot analysis for recombinant TET3 (824-1795) protein. 250 ng of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 ug, 312.5 and 625 ng TET3 protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at room temperature for 1 hour. Sample was concentrated to 5 ul, then 1 ul sample was spotted onto positively charged nylon membrane. 5-hmC antibody was used to detect the generation of products.)
DB (Dot Blot)
(Dot Blot analysis for recombinant TET3 (824-1795) protein. 250 ng of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 ug, 312.5 and 625 ng TET3 protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at room temperature for 1 hour. Sample was concentrated to 5 ul, then 1 ul sample was spotted onto positively charged nylon membrane. 5-hmC antibody was used to detect the generation of products.)
SDS-PAGE
(Recombinant TET3 (824-1795) protein gel. Recombinant TET3 (824-1795) protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant TET3 (824-1795) protein gel. Recombinant TET3 (824-1795) protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for Tet3 recombinant protein
Short Description: Recombinant TET3 (824-1795) protein that includes amino acids 824-1795 of human TET3 protein (accession number NP_001274420.1) was expressed in Sf9 cells and contains an N-terminal 6xHis-tag and DYKDDDDK-Tag with a molecular weight of 111.3 kDa. The recombinant protein is >30% pure by SDS-PAGE. It is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET3 plays an important role in DNA methylation reprogramming processes in the mammalian zygote. TET3 may also contribute to somatic cell nuclear reprogramming during animal cloning. Both of CXXC domain and catalytic domain are important for TET3 regulating key genes of Xenopus eye and neural development.
Background: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET3 plays an important role in DNA methylation reprogramming processes in the mammalian zygote. TET3 may also contribute to somatic cell nuclear reprogramming during animal cloning. Both of CXXC domain and catalytic domain are important for TET3 regulating key genes of Xenopus eye and neural development.
Product Categories/Family for Tet3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
193,705 Da
NCBI Official Full Name
methylcytosine dioxygenase TET3
NCBI Official Synonym Full Names
tet methylcytosine dioxygenase 3
NCBI Official Symbol
TET3
NCBI Official Synonym Symbols
hCG_40738
NCBI Protein Information
methylcytosine dioxygenase TET3
UniProt Protein Name
Methylcytosine dioxygenase TET3
UniProt Gene Name
TET3
UniProt Synonym Gene Names
KIAA0401
UniProt Entry Name
TET3_HUMAN
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Product Notes
The Tet3 tet3 (Catalog #AAA59672) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tet3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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