Rat Myocyte-specific enhancer factor 2C (MEF2C) ELISA Kit | MEF2C elisa kit

Rat Myocyte-specific enhancer factor 2C (MEF2C) ELISA Kit

Cat. Num. AAA27235

• Gene Names: MEF2C; DEL5q14.3; C5DELq14.3
• Reactivity: Rat
• Synonyms: Myocyte-specific enhancer factor 2C (MEF2C); N/A; Rat Myocyte-specific enhancer factor 2C (MEF2C) ELISA Kit; MEF2C elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of MEF2C. No significant cross-reactivity or interference between MEF2C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MEF2C and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MEF2C elisa kit

Principle of the Assay: MEF2C ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MEF2C antibody and an MEF2C-HRP conjugate. The assay sample and buffer are incubated together with MEF2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MEF2C concentration since MEF2C from samples and MEF2C-HRP conjugate compete for the anti-MEF2C antibody binding site. Since the number of sites is limited, as more sites are occupied by MEF2C from the sample, fewer sites are left to bind MEF2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MEF2C concentration in each sample is interpolated from this standard curve.

Intended Uses: This MEF2C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MEF2C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for MEF2C elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 19923215
• NCBI GeneID: 4208
• NCBI Accession #: NP_002388.2
• NCBI GenBank Nucleotide #: NM_002397.4
• Molecular Weight: 50,242 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2C isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2C
• NCBI Official Symbol: MEF2C
• NCBI Official Synonym Symbols: DEL5q14.3; C5DELq14.3
• NCBI Protein Information: myocyte-specific enhancer factor 2C; MADS box transcription enhancer factor 2, polypeptide C
• UniProt Protein Name: Myocyte-specific enhancer factor 2C
• UniProt Gene Name: MEF2C
• UniProt Entry Name: MEF2C_HUMAN