Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA Kit | RANTES/CCL5 elisa kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5, RANTES/CCL5 ELISA Kit

Cat. Num. AAA80628

• Gene Names: Ccl5; Scya5; Rantes
• Reactivity: Rat
• Synonyms: regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5); N/A; Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5, RANTES/CCL5 ELISA Kit; Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit; D17S136E; MGC17164; RANTES; SCYA5; SISd; TCP228; OTTHUMP00000197106; SIS-delta; T-cell specific protein p288; beta-chemokine RANTES; regulated upon activation; normally T-expressed; and presumably secre; RANTES/CCL5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat RANTES/CCL5. No significant cross-reactivity or interference between rat RANTES/CCL5 and analogues was observed.
• Sequence Length: 92
• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for RANTES/CCL5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RANTES/CCL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RANTES/CCL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RANTES/CCL5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RANTES/CCL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 1711393
• NCBI GeneID: 81780
• UniProt Accession #: P50231
• Molecular Weight: 10,170 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: Scya5; Rantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES; regulated upon activation normal T-cell expressed and secreted
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_RAT