RANTES-CCL5 active protein

Mouse RANTES-CCL5 Recombinant

Cat. Num. AAA76394

• Gene Names: Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: RANTES-CCL5; N/A; Mouse RANTES-CCL5 Recombinant; RANTES-CCL5 active protein

• Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant mouse RANTES/CCL5 was lyophilized from a 0.2 um filtered PBS solution.
• Sequence: MKISAAALTI ILTAAALCTP APASPYGSDT TPCCFAYLSL ALPRAHVKEY FYTSSKCSNL AVVFVTRRNR QVCANPEKKW VQEYINYLEM S

• Source: Optimized DNA sequence encoding Mouse RANTES mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: The ED(50) was determined by the ability to chemoattract human lymphocyte population using a concentration range of 0.0-10.0 ng/ml.
• Method: Induction of ORMDL3 and CD48 expressionBM-derived eosinophils (1 × 106/well) were suspended in culture medium alone or medium containing IL-5, IL-3 (both from)ANTES-1 (& all at 100 ng/ml) or eotaxin-1 (100 nM) for 2 h at 37 degree C and 5% CO2 after which cells were analyzed for OML3 mNA expression byT-PC and qPC.; To evaluate CD48 mRNA expression, eosinophils were treated with IL-3 as described above.; For protein expression, eosinophils were treated with IL-3 for 12 h and then examined for ORMDL3 and CD48 by Western blot analysis followed by densitometry using ImageJ (for ORMDL3).
• Molecular Function: Cytokine
• Biological Process: Chemotaxis, Inflammatory-response
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(Validation of the array expression pattern by quantitative RT-PCR.The mRNA levels of CCL2/MCP-1 , CCL5/RANTES , ICAM-1 , VCAM-1 , E-selectin , and P-selectin were determined by quantitative real-time PCR, and values were normalized to 18S rRNA as reference gene.)

Application Data

(Binding and in vitro activity of murine 18V4F hybridoma antibody.Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.)

Application Data

(Binding and in vitro activity of murine 18V4F hybridoma antibody.)

Application Data

(Ccr1 ligands are induced after Candida infection and are chemotactic for kidney neutrophils ex vivo.)

Application Data

(CCL5-induced tumor-promoting properties in CRC cells.Proliferation of the CT26 and HT29 cells was assessed in response to a 5-day treatment with base medium alone (BSA, open bars), with serum-enriched medium (FBS, hatched bars) or with the indicated concentrations of recombinant CCL5 (filled bars), in the presence or in the absence of TAK-779 or anti-CCL5 antibodies (at the indicated concentrations).)

Product Categories/Family for RANTES-CCL5 active protein

Recombinant Mouse Cytokines; CCL chemokines

NCBI and Uniprot Product Information

• NCBI GI #: 164698428
• NCBI GeneID: 20304
• NCBI Accession #: NP_038681.2
• NCBI GenBank Nucleotide #: NM_013653.3
• UniProt Accession #: P30882
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: SISd; Scya5; RANTES; TCP228; MuRantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; T-cell-specific protein RANTES; small inducible cytokine A5; small-inducible cytokine A5
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_MOUSE