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product-image-AAA248549_SC15.jpg Standard Curve (Sample)

Rat ROCK1 (Rhoassociated, coiled-coil containing) ELISA Kit | ROCK1 elisa kit

Rat ROCK1 (Rhoassociated, coiled-coil containing) ELISA Kit

Gene Names
Rock1; ROCK-I; P160Rock
Reactivity
Rat
Synonyms
ROCK1 (Rhoassociated, coiled-coil containing); N/A; Rat ROCK1 (Rhoassociated, coiled-coil containing) ELISA Kit; ROCK1 elisa kit
Ordering
Reactivity
Rat
Sequence Length
1369
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
125-8000pg/ml
Sensitivity
75pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

product-image-AAA248549_SC15.jpg Standard Curve (Sample)
Related Product Information for ROCK1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ROCK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ROCK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ROCK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ROCK1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
114,243 Da
NCBI Official Full Name
Rho-associated protein kinase 1
NCBI Official Synonym Full Names
Rho-associated coiled-coil containing protein kinase 1
NCBI Official Symbol
Rock1
NCBI Official Synonym Symbols
ROCK-I; P160Rock
NCBI Protein Information
rho-associated protein kinase 1
UniProt Protein Name
Rho-associated protein kinase 1
UniProt Gene Name
Rock1
UniProt Synonym Gene Names
ROCK-I; p160ROCK

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Product Notes

The Rat ROCK1 rock1 (Catalog #AAA248549) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA248549 ELISA Kit recognizes Rat ROCK1. It is sometimes possible for the material contained within the vial of "ROCK1 (Rhoassociated, coiled-coil containing), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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