Standard Curve (Sample)
Rat Stress-70 protein, mitochondrial (HSPA9) ELISA Kit | HSPA9 elisa kit
Rat Stress-70 protein, mitochondrial (HSPA9) ELISA Kit
Gene Names
HSPA9; CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
Reactivity
Rat
Synonyms
Stress-70 protein, mitochondrial (HSPA9); N/A; Rat Stress-70 protein, mitochondrial (HSPA9) ELISA Kit; HSPA9 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of HSPA9. No significant cross-reactivity or interference between HSPA9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSPA9 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HSPA9 elisa kit
Intended Uses: This HSPA9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HSPA9. This ELISA kit for research use only!
Principle of the Assay: HSPA9 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSPA9 antibody and an HSPA9-HRP conjugate. The assay sample and buffer are incubated together with HSPA9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPA9 concentration since HSPA9 from samples and HSPA9-HRP conjugate compete for the anti-HSPA9 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPA9 from the sample, fewer sites are left to bind HSPA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPA9 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: HSPA9 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSPA9 antibody and an HSPA9-HRP conjugate. The assay sample and buffer are incubated together with HSPA9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPA9 concentration since HSPA9 from samples and HSPA9-HRP conjugate compete for the anti-HSPA9 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPA9 from the sample, fewer sites are left to bind HSPA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPA9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,680 Da
NCBI Official Full Name
stress-70 protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 70kDa protein 9 (mortalin)
NCBI Official Symbol
HSPA9
NCBI Official Synonym Symbols
CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
NCBI Protein Information
stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m
UniProt Protein Name
Stress-70 protein, mitochondrial
UniProt Gene Name
HSPA9
UniProt Synonym Gene Names
GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
UniProt Entry Name
GRP75_HUMAN
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Product Notes
The Rat HSPA9 hspa9 (Catalog #AAA244896) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244896 ELISA Kit recognizes Rat HSPA9. It is sometimes possible for the material contained within the vial of "Stress-70 protein, mitochondrial (HSPA9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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