Standard Curve (Sample)
(Fig.1. Rat Thrombospondin 1 (TSP-1) Standard Curve.)
Rat Thrombospondin 1 (TSP-1) ELISA Kit | TSP-1 elisa kit
Rat Thrombospondin 1 (TSP-1) ELISA Kit
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Reactivity
Rat
Synonyms
Thrombospondin 1 (TSP-1); N/A; Rat Thrombospondin 1 (TSP-1) ELISA Kit; TSP-1 elisa kit
Reactivity
Rat
Specificity
Rat Thrombospondin 1 (TSP-1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TSP-1. No significant cross-reactivity or interference between Rat TSP-1 and analogues was observed.
Sequence Length
1170
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Thrombospondin 1 (TSP-1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Thrombospondin 1 (TSP-1) Standard Curve.)
Related Product Information for TSP-1 elisa kit
Description: This Rat Thrombospondin 1 (TSP-1) ELISA Kit employs a two-site sandwich ELISA to quantitate TSP-1 in samples. An antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins, and they function to regulate cell adhesion, migration, proliferation, and differentiation. The deduced 242-amino acid protein contains 4 transmembrane domains and other features characteristic of tetraspanins, including conservation of charged residues in or near the transmembrane domains, similar to ion channels, and of 4 cysteines in the second extracellular domain. EST database analysis suggested that TSPAN2 is expressed in colon, endometrium, pancreas, normal and neoplastic prostate, and pregnant uterus.
Background: TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins, and they function to regulate cell adhesion, migration, proliferation, and differentiation. The deduced 242-amino acid protein contains 4 transmembrane domains and other features characteristic of tetraspanins, including conservation of charged residues in or near the transmembrane domains, similar to ion channels, and of 4 cysteines in the second extracellular domain. EST database analysis suggested that TSPAN2 is expressed in colon, endometrium, pancreas, normal and neoplastic prostate, and pregnant uterus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thrombospondin-1
NCBI Official Synonym Full Names
thrombospondin 1
NCBI Official Symbol
THBS1
NCBI Official Synonym Symbols
TSP; THBS; TSP1; TSP-1; THBS-1
NCBI Protein Information
thrombospondin-1
UniProt Protein Name
Thrombospondin-1
UniProt Gene Name
THBS1
UniProt Synonym Gene Names
TSP; TSP1
UniProt Entry Name
TSP1_HUMAN
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Product Notes
The Rat TSP-1 thbs1 (Catalog #AAA330164) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330164 ELISA Kit recognizes Rat TSP-1. It is sometimes possible for the material contained within the vial of "Thrombospondin 1 (TSP-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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