Standard Curve (Sample)
TNF alpha elisa kit
Rat TNF alpha ELISA Kit
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Reactivity
Rat. No cross reactivity with other rat cytokines
Synonyms
TNF alpha; Rat TNF alpha ELISA Kit; TNF-a; TNF alpha elisa kit
Reactivity
Rat. No cross reactivity with other rat cytokines
Sequence Length
233
Samples
Supernatants, buffered solutions or serum and plasma samples
Assay Type
Quantitative Sandwich
Sensitivity
< 15 pg/ml
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNF alpha elisa kit
Intended Uses: The Rat TNFalpha ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of Rat TNFalpha in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Rat TNFalpha.
Principle of the Assay: A capture Antibody highly specific for Rat TNFalpha has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Rat TNFalpha samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-Rat TNFalpha secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Rat TNFalpha present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Rat TNFalpha in any sample tested.
Principle of the Assay: A capture Antibody highly specific for Rat TNFalpha has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Rat TNFalpha samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-Rat TNFalpha secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Rat TNFalpha present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Rat TNFalpha in any sample tested.
Product Categories/Family for TNF alpha elisa kit
References
Aragno M. et al., Endocrinology, 2005; 146(12):5561-7
Chen, G. et al., Ann. Clin. Lab. Sci.,2008; 38(1): 65-74.
Chen, G. et al., Ann. Clin. Lab. Sci.,2008; 38(1): 65-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,644 Da
NCBI Official Full Name
TNF-alpha
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The Rat. No cross reactivity with other rat cytokines TNF alpha tnf (Catalog #AAA13471) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13471 ELISA Kit recognizes Rat. No cross reactivity with other rat cytokines TNF alpha. It is sometimes possible for the material contained within the vial of "TNF alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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