Standard Curve (Sample)
Rat Tyrosine-protein kinase Fyn (FYN) ELISA Kit | FYN elisa kit
Rat Tyrosine-protein kinase Fyn (FYN) ELISA Kit
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Rat
Synonyms
Tyrosine-protein kinase Fyn (FYN); N/A; Rat Tyrosine-protein kinase Fyn (FYN) ELISA Kit; FYN elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of FYN. No significant cross-reactivity or interference between FYN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FYN and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FYN elisa kit
Intended Uses: This FYN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FYN. This ELISA kit for research use only!
Principle of the Assay: FYN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FYN antibody and an FYN-HRP conjugate. The assay sample and buffer are incubated together with FYN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FYN concentration since FYN from samples and FYN-HRP conjugate compete for the anti-FYN antibody binding site. Since the number of sites is limited, as more sites are occupied by FYN from the sample, fewer sites are left to bind FYN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FYN concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FYN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FYN antibody and an FYN-HRP conjugate. The assay sample and buffer are incubated together with FYN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FYN concentration since FYN from samples and FYN-HRP conjugate compete for the anti-FYN antibody binding site. Since the number of sites is limited, as more sites are occupied by FYN from the sample, fewer sites are left to bind FYN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FYN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FYN elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,513 Da
NCBI Official Full Name
tyrosine-protein kinase Fyn isoform a
NCBI Official Synonym Full Names
FYN proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
FYN
NCBI Official Synonym Symbols
SLK; SYN; p59-FYN
NCBI Protein Information
tyrosine-protein kinase Fyn; src-like kinase; proto-oncogene Syn; c-syn protooncogene; proto-oncogene c-Fyn; src/yes-related novel; tyrosine kinase p59fyn(T); FYN oncogene related to SRC, FGR, YES; OKT3-induced calcium influx regulator
UniProt Protein Name
Tyrosine-protein kinase Fyn
UniProt Gene Name
FYN
UniProt Synonym Gene Names
SLK
UniProt Entry Name
FYN_HUMAN
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Product Notes
The Rat FYN fyn (Catalog #AAA246158) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246158 ELISA Kit recognizes Rat FYN. It is sometimes possible for the material contained within the vial of "Tyrosine-protein kinase Fyn (FYN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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