FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD47 antibody mIAP430 (AAA72245). Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of mIAP430 (AAA72245-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rat anti-Human CD47 Antibody | anti-CD47 antibody
Anti-CD47 [mIAP430], Rat IgG2a
Gene Names
CD47; IAP; OA3; MER6
Reactivity
Human
Applications
Immunofluorescence
Purity
Protein A affinity purified
Synonyms
CD47; N/A; Anti-CD47 [mIAP430], Rat IgG2a; Anti-CD47 [mIAP430]; IAP; C5/D5; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; anti-CD47 antibody
Host
Rat
Reactivity
Human
Isotype
Rat IgG2a, Kappa
Clone Number
mIAP430
Specificity
This antibody recognises CD47 which is a a multitransmembrane glycoprotein widely expressed on all hematopoietic cells and most other cell types. It is involved in cell adhesion, Ca2+ influx, platelet activation and peripheral mononuclear neutrophils migration across endothelial and epithelial monolayers.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD47 antibody
Blocking, IF (Immunofluorescence)
Immunogen
This antibody was raised by immunising IAP-deficient mice with purified human placental CD47.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD47 antibody mIAP430 (AAA72245). Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of mIAP430 (AAA72245-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD47 antibody mIAP430 (AAA72245). Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of mIAP430 (AAA72245-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells using anti-CD47 antibody (AAA72245) mIAP430. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of mIAP430 (AAA72245-23.0) at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72245-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells using anti-CD47 antibody (AAA72245) mIAP430. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of mIAP430 (AAA72245-23.0) at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72245-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD47 antibody
This antibody can be used to investigate the role of the CD47 receptor. mIAP430 was first used to visualise the distribution of CD47 on fusing macrophages (Han et al., 2000). Further, it was demonstrated that this antibody can block fusion of macrophages by blocking CD47 and preventing the binding of this receptor with MFR (Han et al., 2000).
Product Categories/Family for anti-CD47 antibody
References
Han et al. CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. J Biol Chem. 2000 Dec 1;275(48):37984-92. PMID:10964914
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
323
NCBI Official Full Name
Leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
NCBI Protein Information
leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
UniProt Entry Name
CD47_HUMAN
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Product Notes
The CD47 cd47 (Catalog #AAA72245) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD47 [mIAP430], Rat IgG2a reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, Blocking, IF (Immunofluorescence). Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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