Collagen Native Protein | COL4A6 native protein
Type I Rat Tendon Collagen-4 mg/ml
Gene Names
COL4A6; DFNX6; DELXq22.3; CXDELq22.3
Applications
Cell Culture
Purity
>95% (SDS-PAGE)
Synonyms
Collagen; N/A; Type I Rat Tendon Collagen-4 mg/ml; COL4A6 native protein
Host
Rat
Purity/Purification
>95% (SDS-PAGE)
Form/Format
Liquid
Concentration
4 mg/ml by Sicrol Assay (varies by lot)
Sequence Length
1678
Applicable Applications for COL4A6 native protein
Cell Culture
Source Note
Rat Tendon
pH
3.0
Buffer
0.2 M Acetic Acid
Gel information
Gel in a few minutes after neutralizing the product
Great for coating plates and T-flasks
Works well when used in 3D tissue models
Great for coating plates and T-flasks
Works well when used in 3D tissue models
Recommended Coating and Washing Volumes
1. Add sufficient collagen solution to coat dishes, plates or inserts. 1-2 ml of solution is sufficient to cover a 35 mm dish. See below table for recommendations for different well sizes.
2. Incubate at room temperature in a biological safety cabinet partially covered for 1 hour.
3. Carefully aspirate remaining solution. Rinse with proper volume of serum-free media to remove buffer solution.
4. Coated plates are best used immediately but can be air-dried and stored.
5. Coated plates that have not been cultured can be dried and stored at 2 -8 degree C and are useful up to 1 week under sterile conditions
Wells: 6; Area(cm2): 4.67; Coating Volume (mL): 0.6; Wash Volume (mL): 1
Wells: 12; Area(cm2): 1.12; Coating Volume (mL): 0.25; Wash Volume (mL): 0.4
Wells: 24; Area(cm2): 0.33; Coating Volume (mL): 0.05; Wash Volume (mL): 0.1
Wells: 75mm insert; Area(cm2): 44; Coating Volume (mL): 5; Wash Volume (mL): 8
Wells: 96; Area(cm2): 0.143; Coating Volume (mL): 0.025; Wash Volume (mL): 0.05
2. Incubate at room temperature in a biological safety cabinet partially covered for 1 hour.
3. Carefully aspirate remaining solution. Rinse with proper volume of serum-free media to remove buffer solution.
4. Coated plates are best used immediately but can be air-dried and stored.
5. Coated plates that have not been cultured can be dried and stored at 2 -8 degree C and are useful up to 1 week under sterile conditions
Wells: 6; Area(cm2): 4.67; Coating Volume (mL): 0.6; Wash Volume (mL): 1
Wells: 12; Area(cm2): 1.12; Coating Volume (mL): 0.25; Wash Volume (mL): 0.4
Wells: 24; Area(cm2): 0.33; Coating Volume (mL): 0.05; Wash Volume (mL): 0.1
Wells: 75mm insert; Area(cm2): 44; Coating Volume (mL): 5; Wash Volume (mL): 8
Wells: 96; Area(cm2): 0.143; Coating Volume (mL): 0.025; Wash Volume (mL): 0.05
Preparation and Storage
Store at 2-8°C.
Related Product Information for COL4A6 native protein
Collagen naturally plays an important role within the body, but it is being used increasingly within certain areas of medicine as well. The breakdown of the body natural collagen can lead to a number of health problems, but the protein can also be used in other contexts to help with the repair of the body. Collagen comprises a family of genetically distinct molecules, all of which have a unique triple helix configuration of three polypeptide subunits known as alpha-chains. Each chain contains around 1000 amino acids, and usually features an amino acid sequence consisting of glycine, proline and hydroxyproline.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
collagen
NCBI Official Synonym Full Names
collagen type IV alpha 6 chain
NCBI Official Symbol
COL4A6
NCBI Official Synonym Symbols
DFNX6; DELXq22.3; CXDELq22.3
NCBI Protein Information
collagen alpha-6(IV) chain
UniProt Protein Name
Collagen alpha-6(IV) chain
UniProt Gene Name
COL4A6
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Product Notes
The COL4A6 col4a6 (Catalog #AAA76590) is a Native Protein produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Collagen can be used in a range of immunoassay formats including, but not limited to, Cell Culture. Researchers should empirically determine the suitability of the COL4A6 col4a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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