Fade Mounting Medium Reagent
Anti-fade Mounting Medium (With DAPI)
Synonyms
Fade Mounting Medium; N/A; Anti-fade Mounting Medium (With DAPI); Fade Mounting Medium reagent
Protocol
1. Balance to room temperature before use.
2. Pipette to remove the liquid on the slide and slightly dry by airing.
3. Pipette 20 ?l of Anti-fade Mounting Medium (With DAPI) and add onto the sample on the slide, or add proper volume of such medium into the cell culture wells directly.
4. Coverslip the slide, but avoid bubbles.
5. Observe under fluorescence microscope. Or store the coverslipped slide at 4°C or -20°C in dark for 2-3 weeks, the fluorescence will not be faded.
2. Pipette to remove the liquid on the slide and slightly dry by airing.
3. Pipette 20 ?l of Anti-fade Mounting Medium (With DAPI) and add onto the sample on the slide, or add proper volume of such medium into the cell culture wells directly.
4. Coverslip the slide, but avoid bubbles.
5. Observe under fluorescence microscope. Or store the coverslipped slide at 4°C or -20°C in dark for 2-3 weeks, the fluorescence will not be faded.
Note
1. The coverslips which were added with this medium must be stored in dark.
2. The medium can definitely slow quenching, but try to observe and take photo asap.
3. Please wear gloves during operating.
2. The medium can definitely slow quenching, but try to observe and take photo asap.
3. Please wear gloves during operating.
Preparation and Storage
Store at 2-8°C for one year
Related Product Information for Fade Mounting Medium reagent
Introduction:
1. With anti-fluorescence quenching reagent, this glycerol-based mounting medium has strong effect on anti-fade and can be used to seal tissues or cells slides in fluorescence related applications. After mounting, coverslipped slides will not readily dry out and can be observed for weeks afterwards without sealing (can be stored at 4°C or -20°C in dark for 2-3 weeks). The medium can prolong the duration of fluorescence and maintain the luminescence intensity of fluorescence maximally, in addition, the other components help to maintain the combination of antigen and antibody.
2. The medium are compatible with a wide array of fluorochromes, enzymatic substrates and fluorescent proteins, such as blue fluorescent protein, FITC, Cy2, Cy3, Cy5, Cy7, DAPI, Rhodamine, Texas Red, AMCA, R6G, Hoechst 33258, Alexa Fluor, etc.
3. DAPI (4',6-diamidino-2-phenylindole, C16H15N5, molecular weight: 350.25, CAS # 28718-90-3) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. DAPI can pass through an intact cell membrane therefore it can be used to stain both live and fixed cells.
4. DAPI is a blue-fluorescent DNA stain that exhibits 20-fold enhancement of fluorescence upon binding to A-T rich regions in DNA. It is excited by the violet laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry and chromosome staining. Because of its high affinity for DNA, it is also frequently used for counting cells, measuring apoptosis, sorting cells based on DNA content, and as a nuclear segmentation tool in high-content imaging analysis.
5. DAPI has an absorption maximum at a wavelength of 340 nm and its emission maximum is at 488 nm, when bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue).
1. With anti-fluorescence quenching reagent, this glycerol-based mounting medium has strong effect on anti-fade and can be used to seal tissues or cells slides in fluorescence related applications. After mounting, coverslipped slides will not readily dry out and can be observed for weeks afterwards without sealing (can be stored at 4°C or -20°C in dark for 2-3 weeks). The medium can prolong the duration of fluorescence and maintain the luminescence intensity of fluorescence maximally, in addition, the other components help to maintain the combination of antigen and antibody.
2. The medium are compatible with a wide array of fluorochromes, enzymatic substrates and fluorescent proteins, such as blue fluorescent protein, FITC, Cy2, Cy3, Cy5, Cy7, DAPI, Rhodamine, Texas Red, AMCA, R6G, Hoechst 33258, Alexa Fluor, etc.
3. DAPI (4',6-diamidino-2-phenylindole, C16H15N5, molecular weight: 350.25, CAS # 28718-90-3) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. DAPI can pass through an intact cell membrane therefore it can be used to stain both live and fixed cells.
4. DAPI is a blue-fluorescent DNA stain that exhibits 20-fold enhancement of fluorescence upon binding to A-T rich regions in DNA. It is excited by the violet laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry and chromosome staining. Because of its high affinity for DNA, it is also frequently used for counting cells, measuring apoptosis, sorting cells based on DNA content, and as a nuclear segmentation tool in high-content imaging analysis.
5. DAPI has an absorption maximum at a wavelength of 340 nm and its emission maximum is at 488 nm, when bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue).
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Product Notes
The Fade Mounting Medium (Catalog #AAA59111) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fade Mounting Medium, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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