Application Data
i-Fect Reagent
i-Fect Transfection Kit
Synonyms
i-Fect; N/A; i-Fect Transfection Kit; siRNA Transfection Kit; i-Fect reagent
Preparation and Storage
4-8 degree C
Application Data
Application Data
Related Product Information for i-Fect reagent
Background: RNA interference (RNAi) is emerging as an effective tool for inhibiting gene expression in a variety of organisms (e.g. plants, insects, and nematodes) and cultured mammalian cell lines. It is characterized by targeted mRNA degradation after introduction of sequence-specific double stranded RNA (dsRNA) into cells. Although cellular uptake of long dsRNA by organisms such as C. elegans and Drosophila has proven to be an effective method to induce RNAi, it tends to result in nonspecific gene suppression in vetebrate cells due in part to interferon response. Recently, it has been discovered that short (less than 30 nucleotides) dsRNA, referred to as small interfering RNAs (siRNA), can cause gene-specific silencing in mammalian cells (1,2,3). In addition, the RNAi effect caused by siRNA can be detectable even after many cell divisions. These properties make siRNA transfection a useful tool for gene silencing in mammalian cells.
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Product Notes
The i-Fect (Catalog #AAA76531) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "i-Fect, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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