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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 4000-4050 of 10000 product results
WB (Western Blot)
(HMT assay for recombinant PRMT5 protein activity 0.25 ug of Histone H4 was incubated with 0 ug, 0.075 ug, 0.15 ug, and 0.3 ug PRMT5 protein in a 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. Reaction products were run on a 12.5% SDS-PAGE and detected by H4R3me2s antibody . PRMT5 only was used as negative control. Western Blot was used for activity detection.)
PRMT5, Recombinant Protein (Cat# AAA59657)
Application Data
(PRMT6 activity assay. 30 ng/ul Recombinant Histone H3.1 or 60 ng/ul Calf Thymus Octamer were incubated with 15 ng/ul PRMT6 (+) and 30 ng/ul PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography.)
PRMT6, Recombinant Protein (Cat# AAA59658)
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K36me2 (MLA). Expected mass: 15271 Da Observed mass: 15271 Da)
Histone H3K36me2, Recombinant Protein (Cat# AAA59775)
Application Data
(HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity 3 uM histone H3K9ac peptide was incubated with different concentrations of HDAC3 / NCOR2 Complex, His-tag in reaction buffer for 30 minutes at 37 degree C. H3K9me0-Eu antibody was used to detect the reaction products.)
HDAC3/NCOR2, Recombinant Protein (Cat# AAA59781)
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K27ME1 (MLA). Expected mass: 15257 Da Observed mass: 15257 Da)
Histone H3K27me1, Recombinant Protein (Cat# AAA59790)
SDS-PAGE
(Recombinant Mononucleosomes H3.3 (R8C) 12.5% SDS-PAGE gel with Coomassie Blue staining MW: 108 kDa Purity: >95%)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60196)
SDS-PAGE
(Recombinant Mononucleosomes H3.3 (K18M) 12.5% SDS-PAGE gel stained with Coomassie Blue MW: 108 kDa Purity: >95%)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60197)
Application Data
(HTRF assay for EGFR (672-1210) protein activity 1 uM TK substrate was incubated with different concentrations of EGFR (672-1210) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hr. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All reactions were performed at room temp. HTRF assay was used for detection.)
EGFR, Recombinant Protein (Cat# AAA60198)
Application Data
(HTRF for IGF2BP1 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP1 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
IGF2BP1, Recombinant Protein (Cat# AAA60209)
SDS-PAGE
(Recombinant SARS-CoV-2 NSP9 protein gel 12.5% SDS-PAGE with Coomassie blue staining MW: 12.38 kDa Purity: >90%)
COVID 19 NSP9 Coronavirus, Recombinant Protein (Cat# AAA60213)
SDS-PAGE
TALDO1, Recombinant Protein (Cat# AAA48619)
SDS-PAGE
MAGEA4, Recombinant Protein (Cat# AAA48620)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
TDO2, Recombinant Protein (Cat# AAA48657)
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
alpha-Synuclein, Recombinant Protein (Cat# AAA48667)
SDS-PAGE
uFM1, Recombinant Protein (Cat# AAA48668)
SDS-PAGE
TSN, Recombinant Protein (Cat# AAA48679)
BDH2, Recombinant Protein (Cat# AAA48693)
SDS-PAGE
PLAC8, Recombinant Protein (Cat# AAA48847)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
GLuD1, Recombinant Protein (Cat# AAA48857)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualization by coomassie blue stain.)
CD8B, Recombinant Protein (Cat# AAA48869)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
HP, Recombinant Protein (Cat# AAA48876)
SDS-PAGE
NRGN, Recombinant Protein (Cat# AAA48877)
SDS-PAGE
CNPY1, Recombinant Protein (Cat# AAA48883)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
BRAF, Recombinant Protein (Cat# AAA48888)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SERPINB3, Recombinant Protein (Cat# AAA48901)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
CADM1, Recombinant Protein (Cat# AAA48903)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
IL1a, Recombinant Protein (Cat# AAA48906)
SDS-PAGE
BAIAP2, Recombinant Protein (Cat# AAA48763)
SDS-PAGE
GSTA4, Recombinant Protein (Cat# AAA48777)
SDS-PAGE
MGP, Recombinant Protein (Cat# AAA48785)
SDS-PAGE
CD226, Recombinant Protein (Cat# AAA48790)
SDS-PAGE
BGN, Recombinant Protein (Cat# AAA48793)
SDS-PAGE
TRIM28, Recombinant Protein (Cat# AAA48804)
SDS-PAGE
XPA, Recombinant Protein (Cat# AAA48809)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
NHLH1, Recombinant Protein (Cat# AAA48811)
SDS-PAGE
IGFBP7, Recombinant Protein (Cat# AAA48815)
SDS-PAGE
CPOX, Recombinant Protein (Cat# AAA48827)
SDS-PAGE
MBP, Recombinant Protein (Cat# AAA48833)
SDS-PAGE
CEACAM7, Recombinant Protein (Cat# AAA48837)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
mug, Recombinant Protein (Cat# AAA48842)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
ABRACL, Recombinant Protein (Cat# AAA48846)
SDS-PAGE
(3ug by SDS-Page under reducing condition and visualized by coomassie blue stain.)
DSCR1, Recombinant Protein (Cat# AAA48471)
SDS-PAGE
CIB1, Recombinant Protein (Cat# AAA48478)
SDS-PAGE
Cellubrevin, Recombinant Protein (Cat# AAA48485)
SDS-PAGE
GADD153, Recombinant Protein (Cat# AAA48498)
SDS-PAGE
MAT2A, Recombinant Protein (Cat# AAA48511)
SDS-PAGE
GRB2, Recombinant Protein (Cat# AAA48525)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Chromogranin-A, Recombinant Protein (Cat# AAA48537)
SDS-PAGE
TCL1A, Recombinant Protein (Cat# AAA48561)
SDS-PAGE
Cysteine dioxygenase, Recombinant Protein (Cat# AAA48562)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.