SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
RNASE2 recombinant protein
Recombinant human RNASE2 protein
Gene Names
RNASE2; EDN; RAF3; RNS2
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
RNASE2; N/A; Recombinant human RNASE2 protein; ribonuclease A family member 2; EDN; RAF3; RNS2; non-secretory ribonuclease; Eosinophil-derived neurotoxin; RNase UpI-2; Ribonuclease 2; RNase 2; Ribonuclease US; RNASE2 recombinant protein
Host
Baculovirus
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
28-161aa
Sequence
ADPKPPQFTW AQWFETQHIN MTSQQCTNAM QVINNYQRRC KNQNTFLLTT FANVVNVCGN PNMTCPSNKT RKNCHHSGSQ VPLIHCNLTT PSPQNISNCR YAQTPANMFY IVACDNRDQR RDPPQYPVVP VHLDRIIHHH HHH
Applicable Applications for RNASE2 recombinant protein
SDS-PAGE
Species
Human
Tag
His-Tag
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for RNASE2 recombinant protein
RNASE2, is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein is found in eosinophil granulocytes and closely related to the eosinophil cationic protein (RNASE3) from which it diverged 50 million years ago after the split between the old world and the new world monkeys. It is relatively neutral and has cytotoxic properties. It may generate host defense via roles in promoting leukocyte activation, maturation, and chemotaxis. It has also been studied in the context of defense against pathogen infection in mammals. Recombinant human RNASE2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for RNASE2 recombinant protein
References
Rosenberg HF., et al, (2015) Int J Mol Sci. 14:15442-15455. ;; Levin AM., et al, (2019) J Allergy Clin Immunol. 143:1791-1802.;; Ostendorf T., et al, (2020) Immunity. 52:591-605.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16.5kDa (143aa)
NCBI Official Full Name
non-secretory ribonuclease
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
NCBI Official Symbol
RNASE2
NCBI Official Synonym Symbols
EDN; RAF3; RNS2
NCBI Protein Information
non-secretory ribonuclease; RNase 2; RNase UpI-2; ribonuclease 2; ribonuclease US; ribonuclease A F3; eosinophil-derived neurotoxin
UniProt Protein Name
Non-secretory ribonuclease
UniProt Gene Name
RNASE2
UniProt Synonym Gene Names
EDN; RNS2; RNase 2
UniProt Entry Name
RNAS2_HUMAN
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Product Notes
The RNASE2 rnase2 (Catalog #AAA48414) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 28-161aa. AAA Biotech's RNASE2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RNASE2 rnase2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPKPPQFTW AQWFETQHIN MTSQQCTNAM QVINNYQRRC KNQNTFLLTT FANVVNVCGN PNMTCPSNKT RKNCHHSGSQ VPLIHCNLTT PSPQNISNCR YAQTPANMFY IVACDNRDQR RDPPQYPVVP VHLDRIIHHH HHH. It is sometimes possible for the material contained within the vial of "RNASE2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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