Application Data
RNASE4 recombinant protein
Human RNASE4 Protein, hFc Tag
Gene Names
RNASE4; RAB1; RNS4
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
RNASE4; N/A; Human RNASE4 Protein, hFc Tag; RAB1; RNS4; RNASE4 recombinant protein
Host
HEK293
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Concentration
0.893 mg/mL (varies by lot)
Tag
C-Human Fc tag
Molecular Characterization
Mouse RNASE4(Gln30-Arg148) 6×His tag
Reconstitution
1.Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample to sttle down at the bottom of the tube.
2.Dissolved lyophilized protein sample in sterile water based on the recommended volume 55.99 uL.
3.After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
2.Dissolved lyophilized protein sample in sterile water based on the recommended volume 55.99 uL.
3.After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
Preparation and Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). For long term storage, glycerol can be added to a final concentration of 50% and stored at -20°C.
Application Data
Application Data
SDS-PAGE
(Figure 1. Human RNASE4 Protein, mFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human RNASE4 Protein, mFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for RNASE4 recombinant protein
Recombinant human RNASE4 protein with C-terminal human Fc tag
The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]
The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]
Product Categories/Family for RNASE4 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
The protein has a predicted molecular mass of 14.7 kDa after removal of the signal peptide. The apparent molecular mass of mRNASE4-His is approximately 10-15 kDa due to glycosylation.
NCBI Official Full Name
Ribonuclease 4
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 4
NCBI Official Symbol
RNASE4
NCBI Official Synonym Symbols
RAB1; RNS4
NCBI Protein Information
ribonuclease 4; RNase 4; ribonuclease A B1
UniProt Protein Name
Ribonuclease 4
UniProt Gene Name
RNASE4
UniProt Synonym Gene Names
RNS4; RNase 4
UniProt Entry Name
RNAS4_HUMAN
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Product Notes
The RNASE4 rnase4 (Catalog #AAA47539) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RNASE4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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