SDS-PAGE
(Figure 1. Human SCUBE2 (441-659) Protein, hFc Tag on SDS-PAGE under reducing condition.)
SCUBE2 recombinant protein
Human SCUBE2(441-659) Protein, hFc Tag
Gene Names
SCUBE2; CEGB1; CEGF1; CEGP1
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
SCUBE2; N/A; Human SCUBE2(441-659) Protein, hFc Tag; CEGP1; Scube/You; SCUBE2 recombinant protein
Host
HEK293
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Concentration
0.328 mg/mL (varies by lot)
Tag
hFc tag
Visual Inspection
Test: Lyopphilization ; Specification: Dried white powder ; Result : Pass
Test: Vial label ; Specification: Label alligned and complete ; Result : Pass
Test: Bottle Sealing (manual check) ; Specification: Sealed tight ; Result: Pass
Test: Vial label ; Specification: Label alligned and complete ; Result : Pass
Test: Bottle Sealing (manual check) ; Specification: Sealed tight ; Result: Pass
Quantity
Test: Bradford Method ; Specification: > Spcified quantity ; Result: Pass
Other Properties
Test: pH ; Specification: 7.4 ± 0.5 ; Result: Pass
Reconsitution
1) Before opening the cap, centrifuge the sample tube at 5000g for 3-5 min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.
2) Dissolved lyophilized sample in sterile and low endotoxin water based on the above concentration, e.g. use 281.69 ul (100/0.355)water to disolve 100ug lyophilized product.
3)After adding sterile water , cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette the sample.
2) Dissolved lyophilized sample in sterile and low endotoxin water based on the above concentration, e.g. use 281.69 ul (100/0.355)water to disolve 100ug lyophilized product.
3)After adding sterile water , cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Molecular Characterization
SCUBE2(Asp441-Asn659 ) hFc(Glu99-Ala330)
Preparation and Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
SDS-PAGE
(Figure 1. Human SCUBE2 (441-659) Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human SCUBE2 (441-659) Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for SCUBE2 recombinant protein
Recombinant human SCUBE2(441-659) protein with C-terminal human Fc tag
Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed:22902404, PubMed:22677548). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687).
Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed:22902404, PubMed:22677548). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687).
Product Categories/Family for SCUBE2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Weight The protein has a predicted molecular mass of 50.2 kDa after removal of the signal peptide. The apparent molecular mass of SCUBE2(441-659)-hFc is approximately 35-55 kDa due to glycosylation.
NCBI Official Full Name
signal peptide, CUB and EGF-like domain-containing protein 2 isoform 1
NCBI Official Synonym Full Names
signal peptide, CUB domain, EGF-like 2
NCBI Official Symbol
SCUBE2
NCBI Official Synonym Symbols
CEGB1; CEGF1; CEGP1
NCBI Protein Information
signal peptide, CUB and EGF-like domain-containing protein 2
UniProt Protein Name
Signal peptide, CUB and EGF-like domain-containing protein 2
UniProt Gene Name
SCUBE2
UniProt Synonym Gene Names
CEGP1
UniProt Entry Name
SCUB2_HUMAN
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Product Notes
The SCUBE2 scube2 (Catalog #AAA47547) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SCUBE2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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