IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human SCUBE2 Polyclonal Antibody | anti-SCUBE2 antibody
SCUBE2 Antibody (Center N266)
Gene Names
SCUBE2; CEGB1; CEGF1; CEGP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SCUBE2, Antibody; SCUBE2 Antibody (Center N266); Signal peptide; CUB and EGF-like domain-containing protein 2; Protein CEGP1; SCUBE2; CEGP1; anti-SCUBE2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SCUBE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the Central region of human SCUBE2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.33 (varies by lot)
Sequence Positions
251-280
Sequence Length
807
Applicable Applications for anti-SCUBE2 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(SCUBE2 Antibody (Center N266) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SCUBE2 Antibody (Center N266) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, ZR-75-1, HT-29 cell line (from left to right), using SCUBE2 Antibody (Center N266). AAA285650 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, ZR-75-1, HT-29 cell line (from left to right), using SCUBE2 Antibody (Center N266). AAA285650 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Product Categories/Family for anti-SCUBE2 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Tsai, M.T., et al. Biochem. J. 422(1):119-128(2009)
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Tsai, M.T., et al. Biochem. J. 422(1):119-128(2009)
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109966
NCBI Official Full Name
signal peptide, CUB and EGF-like domain-containing protein 2 isoform 2
NCBI Official Synonym Full Names
signal peptide, CUB domain, EGF-like 2
NCBI Official Symbol
SCUBE2
NCBI Official Synonym Symbols
CEGB1; CEGF1; CEGP1
NCBI Protein Information
signal peptide, CUB and EGF-like domain-containing protein 2
UniProt Protein Name
Signal peptide, CUB and EGF-like domain-containing protein 2
UniProt Gene Name
SCUBE2
UniProt Synonym Gene Names
CEGP1
UniProt Entry Name
SCUB2_HUMAN
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Product Notes
The SCUBE2 scube2 (Catalog #AAA285650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 251-280. The SCUBE2 Antibody (Center N266) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SCUBE2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the SCUBE2 scube2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCUBE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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